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在国家同步辐射光源下进行生物分子溶液 X 射线散射研究。

Biomolecular solution X-ray scattering at the National Synchrotron Light Source.

机构信息

National Synchrotron Light Source, Brookhaven National Laboratory, Upton, NY 11973, USA.

出版信息

J Synchrotron Radiat. 2011 Jan;18(1):41-4. doi: 10.1107/S0909049510036022. Epub 2010 Nov 5.

DOI:10.1107/S0909049510036022
PMID:21169689
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3004252/
Abstract

In recent years there has been a growing interest in the application of X-ray scattering techniques to biomolecules in solution. At NSLS, a new undulator-based beamline, X9, has been constructed to address the oversubscribed user demand for X-ray scattering. Beamline X9 has the capability to perform small/wide-angle X-ray scattering (SAXS/WAXS) all in one single instrument. This is accomplished by utilizing a vacuum sample/detector chamber that is an integral part of the SAXS scattering flight path. This vacuum chamber allows a WAXS detector to be positioned at a close distance from the sample, while not interfering with scattered X-rays at small angles from reaching the SAXS detector. A regular training program, the X9 workbench, has also been established to allow users to become familiar with beamline X9 for solution X-ray scattering.

摘要

近年来,人们对将 X 射线散射技术应用于溶液中的生物分子越来越感兴趣。在 NSLS,一条新的基于波荡器的光束线 X9 已经建成,以满足用户对 X 射线散射的过度需求。X9 光束线具有在单个仪器中进行小角/广角 X 射线散射(SAXS/WAXS)的能力。这是通过利用真空样品/探测器室来实现的,该室是 SAXS 散射飞行路径的一个组成部分。这个真空室允许 WAXS 探测器被放置在离样品很近的位置,同时不会干扰小角度散射的 X 射线到达 SAXS 探测器。一个定期的培训计划,X9 工作台,也已经建立,以允许用户熟悉用于溶液 X 射线散射的 X9 光束线。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/070a/3004252/97b5df622f6d/s-18-00041-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/070a/3004252/d9484669821e/s-18-00041-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/070a/3004252/0750afa6bc81/s-18-00041-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/070a/3004252/11fdeea8e55c/s-18-00041-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/070a/3004252/ec803dfa381f/s-18-00041-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/070a/3004252/97b5df622f6d/s-18-00041-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/070a/3004252/d9484669821e/s-18-00041-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/070a/3004252/0750afa6bc81/s-18-00041-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/070a/3004252/11fdeea8e55c/s-18-00041-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/070a/3004252/ec803dfa381f/s-18-00041-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/070a/3004252/97b5df622f6d/s-18-00041-fig5.jpg

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