Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Daneshgah Street, Shahid Shiroudi Boulevard, PO-Box: 6714869914, Kermanshah, Iran.
Department of Immunology, School of Medicine, Tarbiat Modares University of Medical Sciences, Tehran, Iran.
Med Microbiol Immunol. 2023 Jun;212(3):221-229. doi: 10.1007/s00430-023-00764-x. Epub 2023 Jun 13.
Pattern recognition receptors of the innate immune system, such as RIG-I and MDA5, are responsible for recognizing viruses and inducing interferon production. Genetic polymorphisms in the coding regions of RLR may be associated with the severity of COVID-19. Considering the contribution of the RLR signaling in immune-mediated reactions, this study investigated the association between three SNP in the coding region of IFIH1 and DDX58 genes with the susceptibility to COVID-19 in the Kermanshah population, Iran. 177 patients with severe and 182 with mild COVID-19 were admitted for this study. Genomic DNA was extracted from peripheral blood leukocytes of patients to determine the genotypes of two SNPs, rs1990760(C>T) and rs3747517(T>C) IFIH1 gene and rs10813831(G>A) DDX58 gene using PCR-RFLP method. Our results showed that the frequency of the AA genotype of rs10813831(G>A) was associated with susceptibility to COVID-19 compared to the GG genotype (p = 0.017, OR = 2.593, 95% CI 1.173-5.736). We also observed a statistically significant difference in the recessive model for SNPs rs10813831 variant (AA versus GG + GA, p = 0.003, OR = 2.901, 95% CI 1.405-6.103). Furthermore, No significant association was found between rs1990760 (C>T) and rs3747517(T>C) of IFIH1 gene polymorphisms with COVID-19. Our findings suggest that DDX58 rs10813831(A>G) polymorphism may be associated with COVID-19 severity in the Kermanshah population, Iran.
先天免疫系统的模式识别受体，如 RIG-I 和 MDA5，负责识别病毒并诱导干扰素的产生。RLR 编码区的遗传多态性可能与 COVID-19 的严重程度有关。考虑到 RLR 信号在免疫介导的反应中的贡献，本研究调查了编码区三个 SNP 与 COVID-19 易感性的关联IFIH1和DDX58基因在伊朗克尔曼沙阿人群中。177 名重症和 182 名轻症 COVID-19 患者入组本研究。从患者外周血白细胞中提取基因组 DNA，采用 PCR-RFLP 法检测两个 SNP 的基因型IFIH1基因(rs1990760C>T 和 rs3747517T>C)和DDX58基因(rs10813831G>A)。我们的结果表明，与 GG 基因型相比，rs10813831(G>A)的 AA 基因型与 COVID-19 的易感性相关(p=0.017，OR=2.593，95%CI 1.173-5.736)。我们还观察到 rs10813831 变异的隐性模型在 SNP 中存在统计学显著差异(AA 与 GG+GA，p=0.003，OR=2.901，95%CI 1.405-6.103)。此外，IFIH1 基因多态性 rs1990760(C>T)和 rs3747517(T>C)与 COVID-19 无显著相关性。我们的研究结果表明，DDX58 rs10813831(A>G)多态性可能与伊朗克尔曼沙阿人群 COVID-19 的严重程度有关。
