**和** 对红莲型细胞质雄性不育（CMS）水稻（*L. ssp.*）恢复系育性恢复的影响。 需注意，原文中“and”前后内容缺失，以上译文根据现有内容尽量准确翻译。
The effects of and on fertility restoration in Honglian-type cytoplasmic male sterile (CMS) lines of rice ( L. ssp. ).
作者信息
Zhang Honggen, Wang Ruixuan, Xu Zuopeng, Zhao Xiangqiang, Gao Hailin, Liu Qiaoquan, Tang Shuzhu
机构信息
Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Agricultural College of Yangzhou University, Yangzhou, 225009 China.
Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009 China.
出版信息
Mol Breed. 2021 Oct 7;41(10):64. doi: 10.1007/s11032-021-01256-7. eCollection 2021 Oct.
UNLABELLED
Honglian (HL)-type cytoplasmic male sterility (CMS) has only been used in the development of three-line rice hybrids, and the fertility of HL-type CMS lines can be restored by two non-allelic fertility-restorer () genes, and . For the development of HL-type hybrid combinations, it is therefore necessary to determine whether and can restore the fertility of HL-type CMS lines. Here, we genetically characterized HL-type CMS lines and the ability of and to restore fertility for breeding HL-type hybrids. I-KI pollen staining revealed that HL-type CMS lines and their derived testcross F hybrids had stained abortive pollen grains, unlike HL-type CMS lines. Crossing experiments showed that and partially restored the fertility of HL-type CMS lines, and showed higher restorability than Furthermore, we found that there were additive and dosage effects of and with respect to fertility restoration in HL-type CMS lines. These results give critical insight into the breeding of HL-type CMS lines and restorers, which will be helpful for the development of commercial HL-type hybrids.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s11032-021-01256-7.
未标注
红莲（HL）型细胞质雄性不育（CMS）仅用于三系杂交水稻的培育，HL型CMS系的育性可由两个非等位育性恢复基因Rf5和Rf6恢复。因此，对于HL型杂交组合的培育，有必要确定Rf5和Rf6是否能恢复HL型CMS系的育性。在此，我们对HL型CMS系进行了遗传特征分析，并研究了Rf5和Rf6恢复HL型杂交种育性的能力。碘-碘化钾花粉染色显示，与HL型可育系不同，HL型CMS系及其衍生的测交F1杂种有染败花粉粒。杂交试验表明，Rf5和Rf6能部分恢复HL型CMS系的育性，且Rf5的恢复能力高于Rf6。此外，我们发现Rf5和Rf6在HL型CMS系育性恢复方面存在加性和剂量效应。这些结果为HL型CMS系和恢复系的选育提供了关键见解，将有助于商业化HL型杂交种的培育。
补充信息
在线版本包含可在10.1007/s11032-021-01256-7获取的补充材料。
Zotero 插件