Fan Fengfeng, Li Nengwu, Chen Yunping, Liu Xingdan, Sun Heng, Wang Jie, He Guangcun, Zhu Yingguo, Li Shaoqing
State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, Engineering Research Center for Plant Biotechnology and Germplasm Utilization of Ministry of Education, College of Life Science, Wuhan UniversityWuhan, China.
College of Agronomy, Hunan Agricultural UniversityChangsha, China.
Front Plant Sci. 2017 Jun 7;8:986. doi: 10.3389/fpls.2017.00986. eCollection 2017.
Hybrid rice has contributed significantly to the world food security. Breeding of elite high-yield, strong-resistant broad-spectrum restorer line is an important strategy for hybrid rice in commercial breeding programs. Here, we developed three elite brown planthopper (BPH)-resistant wide-spectrum restorer lines by pyramiding big-panicle gene , BPH-resistant genes and , fertility restorer genes , and through molecular marker assisted selection. Resistance analysis revealed that the newly developed restorer lines showed stronger BPH-resistance than any of the single-gene donor parent Luoyang-6 and Luoyang-9. Moreover, the three new restorer lines had broad spectrum recovery capabilities for Honglian CMS, Wild abortive CMS and two-line GMS sterile lines, and higher grain yields than that of the recurrent parent 9,311 under nature field conditions. Importantly, the hybrid crosses also showed good performance for grain yield and BPH-resistance. Thus, the development of elite BPH-resistant wide-spectrum restorer lines has a promising future for breeding of broad spectrum BPH-resistant high-yield varieties.
杂交水稻为世界粮食安全做出了重大贡献。培育优良的高产、强抗性广谱恢复系是杂交水稻商业育种计划中的一项重要策略。在此，我们通过分子标记辅助选择，将大穗基因、抗褐飞虱基因和、育性恢复基因和进行聚合，培育出了三个优良的抗褐飞虱广谱恢复系。抗性分析表明，新培育的恢复系表现出比任何单基因供体亲本洛阳6号和洛阳9号更强的抗褐飞虱能力。此外，这三个新恢复系对红莲型细胞质雄性不育系、野败型细胞质雄性不育系和两系光温敏雄性不育系具有广谱恢复能力，且在自然田间条件下比轮回亲本9311具有更高的产量。重要的是，杂交组合在产量和抗褐飞虱方面也表现良好。因此，优良抗褐飞虱广谱恢复系的培育在广谱抗褐飞虱高产品种的育种中具有广阔的前景。
