Establishment of an efficient -mediated genetic transformation system in halophyte .

UNLABELLED

Alkaligrass () is a monocotyledonous halophyte pasture, which has strong tolerance to saline-alkali, drought, and chilling stresses. We have reported a high-quality chromosome-level genome and stress-responsive proteomic results in . However, the gene/protein function investigations are still lacking, due to the absent of genetic transformation system in . In this study, we established a higher efficient -mediated transformation for using calluses induced from seeds. strain EHA105 harbors pANIC 6B vectors that contain reporter gene and gene for screening. Ten mg·L hygromycin was used for selecting transgenic calluses. The optimized condition of vacuum for 10 min, ultrasonication for 10 min, and then vacuum for 10 min was used for improvement of conversion efficiency. Besides, 300 mg·L timentin was the optimum antibiotics in transformation. PCR amplification exhibited that gene has been successfully integrated into the chromosome of . Histochemical GUS staining and qRT-PCR analysis indicated that gene has stably expressed with ß-glucuronidase activity in transgene seedlings. All these demonstrated that we have successfully established an -mediated transformation system of , which provides a good platform for further gene function analysis and lays a solid foundation for molecular breeding.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s11032-021-01247-8.