Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.
Key Laboratory of Saline-alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, 150040, China.
Sci China Life Sci. 2020 Sep;63(9):1269-1282. doi: 10.1007/s11427-020-1662-x. Epub 2020 Mar 12.
Alkaligrass (Puccinellia tenuiflora) is a monocotyledonous halophytic forage grass widely distributed in Northern China. It belongs to the Gramineae family and shares a close phylogenetic relationship with the cereal crops, wheat and barley. Here, we present a high-quality chromosome-level genome sequence of alkaligrass assembled from Illumina, PacBio and 10× Genomics reads combined with genome-wide chromosome conformation capture (Hi-C) data. The ∼1.50 Gb assembled alkaligrass genome encodes 38,387 protein-coding genes, and 54.9% of the assembly are transposable elements, with long terminal repeats being the most abundant. Comparative genomic analysis coupled with stress-treated transcriptome profiling uncovers a set of unique saline- and alkaline-responsive genes in alkaligrass. The high-quality genome assembly and the identified stress related genes in alkaligrass provide an important resource for evolutionary genomic studies in Gramineae and facilitate further understanding of molecular mechanisms underlying stress tolerance in monocotyledonous halophytes. The alkaligrass genome data is freely available at http://xhhuanglab.cn/data/alkaligrass.html .
碱茅草（Puccinellia tenuiflora）是一种广泛分布于中国北方的单子叶盐生牧草。它属于禾本科，与小麦和大麦等谷类作物有着密切的系统发育关系。在这里，我们展示了一种来自 Illumina、PacBio 和 10× Genomics 读取的高质量染色体水平的碱茅草基因组序列，该序列与全基因组染色体构象捕获（Hi-C）数据相结合。组装后的碱茅草基因组约为 1.50 Gb，编码 38387 个蛋白编码基因，组装体的 54.9%是转座元件，其中长末端重复序列最为丰富。比较基因组分析结合受胁迫处理的转录组分析，揭示了碱茅草中一组独特的盐和碱性响应基因。高质量的基因组组装和鉴定的碱茅草胁迫相关基因为禾本科进化基因组研究提供了重要资源，并有助于进一步理解单子叶盐生植物的胁迫耐受分子机制。碱茅草基因组数据可在 http://xhhuanglab.cn/data/alkaligrass.html 免费获取。
