Key Laboratory of Dryland Agriculture, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Key laboratory of Molecular Epigenetics of Ministry of Education (MOE), Northeast Normal University, Changchun, 130024, China.
BMC Genomics. 2020 Apr 19;21(1):311. doi: 10.1186/s12864-020-6727-5.
Puccinellia tenuiflora, a forage grass, is considered a model halophyte given its strong tolerance for multiple stress conditions and its close genetic relationship with cereals. This halophyte has enormous values for improving our understanding of salinity tolerance mechanisms. The genetic information of P. tenuiflora also is a potential resource that can be used for improving the salinity tolerance of cereals.
Here, we sequenced and assembled the P. tenuiflora genome (2n = 14) through the combined strategy of Illumina, PacBio, and 10× genomic technique. We generated 43.2× PacBio long reads, 123.87× 10× genomic reads, and 312.6× Illumina reads. Finally, we assembled 2638 scaffolds with a total size of 1.107 Gb, contig N50 of 117 kb, and scaffold N50 of 950 kb. We predicted 39,725 protein-coding genes, and identified 692 tRNAs, 68 rRNAs, 702 snRNAs, 1376 microRNAs, and 691 Mb transposable elements.
We deposited the genome sequence in NCBI and the Genome Warehouse in National Genomics Data Center. Our work may improve current understanding of plant salinity tolerance, and provides extensive genetic resources necessary for improving the salinity and drought tolerance of cereals.
作为一种饲用盐生植物，中华补血草（Puccinellia tenuiflora）因其对多种胁迫条件的强耐受性以及与谷类作物的密切遗传关系而被认为是一种模式盐生植物。这种盐生植物对于提高我们对耐盐机制的理解具有巨大的价值。中华补血草的遗传信息也是一种潜在的资源，可用于提高谷类作物的耐盐性。
我们通过 Illumina、PacBio 和 10×基因组技术的组合策略对中华补血草（2n = 14）进行了测序和组装。我们生成了 43.2×PacBio 长读长、123.87×10×基因组读长和 312.6×Illumina 读长。最终，我们组装了 2638 个支架，总大小为 1.107Gb，contig N50 为 117kb，scaffold N50 为 950kb。我们预测了 39725 个蛋白编码基因，并鉴定了 692 个 tRNA、68 个 rRNA、702 个 snRNA、1376 个 microRNA 和 691Mb 转座元件。
我们将基因组序列提交到了 NCBI 和国家基因组数据中心的基因组仓库。我们的工作可能会提高人们对植物耐盐性的现有认识，并为提高谷类作物的耐盐性和耐旱性提供广泛的遗传资源。
