Koga T, Nakano S, Nakayama M, Kounoue E, Nagafuchi S, Niho Y, Yamada H
Cancer Res. 1986 Sep;46(9):4431-7.
Medium conditioned by exposure to density-inhibited, tumorigenic V79 Chinese hamster cell cultures reversibly inhibited the growth and DNA synthesis of sparse, proliferating cultures, not only of the same cell line but also of the BALB/c 3T3 A31 murine cell line. This species nonspecific inhibitory activity was found to be mediated by the soluble inhibitor produced endogenously by V79 cells at the time of density inhibition. The molecular weight of this inhibitor is approximately 2000, and production of this compound seems to be serum dependent. Partial purification was done by reverse-phase fast protein liquid chromatography. The inhibitory activity was linearly dependent on the concentration in the inhibitory fraction. This partially purified inhibitor did not include lactic acid, a growth-inhibitory metabolite. These data indicate that a growth-regulatory factor is also operant in tumorigenic V79 cells and suggest that growth of neoplastic cells cannot only be explained by an enhanced positive growth potential but rather by the balance between a positive and negative growth potential.
暴露于密度抑制的致瘤性V79中国仓鼠细胞培养物所条件化的培养基，可逆转地抑制稀疏的、增殖性培养物的生长和DNA合成，这些培养物不仅来自同一细胞系，也来自BALB/c 3T3 A31小鼠细胞系。发现这种种属非特异性抑制活性是由V79细胞在密度抑制时内源性产生的可溶性抑制剂介导的。这种抑制剂的分子量约为2000，该化合物的产生似乎依赖于血清。通过反相快速蛋白质液相色谱法进行了部分纯化。抑制活性与抑制级分中的浓度呈线性相关。这种部分纯化的抑制剂不包括乳酸，一种生长抑制性代谢物。这些数据表明，一种生长调节因子在致瘤性V79细胞中也起作用，并表明肿瘤细胞的生长不能仅通过增强的正生长潜能来解释，而应由正生长潜能和负生长潜能之间的平衡来解释。
