Djerassi I
J Med. 1983;14(5-6):415-32.
A small molecule in tissue culture supernatants has growth inhibitory effects on the parent and other cells. Supernatants from stationary phase cultures of a human normal lymphoid cell line (No. 802) inhibited the growth of the parent cells even when the latter were in optimal concentrations. The inhibitory activity was associated with small molecular weight fractions prepared by filtration. Fractions with materials smaller than 5000 daltons were the most active. Growth inhibition was observed also with fractions smaller than 2000 and 1000 daltons. Similar growth inhibition of parent cells was observed with the small molecular weight fractions of supernatants from the human acute lymphoblastic leukemia cell line CCRF-SB, the human lung Ca cell line A-549 and from a mouse leukemia L1210 line. The latter were inhibited only in the absence of serum. The fractions from all 3 human cell lines inhibited the cells from all human lines indicating a lack of tissue specificity. The fractions from the human 802 line and the mouse L1210 line did not inhibit the cell growth of each other. Species specificity, however, cannot as yet be considered established.
组织培养上清液中的一种小分子对亲代细胞和其他细胞具有生长抑制作用。人正常淋巴细胞系（802号）稳定期培养物的上清液即使在亲代细胞处于最佳浓度时也能抑制其生长。抑制活性与通过过滤制备的小分子部分有关。分子量小于5000道尔顿的部分活性最强。分子量小于2000道尔顿和1000道尔顿的部分也观察到生长抑制。用人急性淋巴细胞白血病细胞系CCRF-SB、人肺癌细胞系A-549和小鼠白血病L1210系的上清液的小分子部分对亲代细胞也观察到类似的生长抑制。后者仅在无血清时受到抑制。来自所有3种人类细胞系的部分抑制了所有人类细胞系的细胞生长，表明缺乏组织特异性。来自人802系和小鼠L1210系的部分不抑制彼此的细胞生长。然而，物种特异性尚未确定。
