日粮中锶雷尼酸和氧化铈添加对肉鸡骨质量和骨钙素、碱性磷酸酶基因表达水平的影响。

Effect of strontium ranelate and cerium oxide addition in the diet on bone quality and expression level of osteocalcin and alkaline phosphatase genes in broiler chicken.

机构信息

Department of Animal Resource, College of Agriculture Engineering Sciences, Salahaddin University-Erbil, Erbil, Iraq.

出版信息

Vet Med Sci. 2023 Jul;9(4):1726-1736. doi: 10.1002/vms3.1190. Epub 2023 Jun 13.

DOI:10.1002/vms3.1190

PMID:37311971

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10357239/

Abstract

BACKGROUND

In the modern broiler industry, leg and gait disorders are considerable problems. Fast-growing broilers are especially susceptible to bone abnormalities, causing major problems for broiler producers. Strontium ranelate (SrR) has been used successfully for the treatment of osteoporosis in humans. In addition, cerium oxide (CeO) is an anti-stress agent applied in the biological system.

METHODS

This study was conducted to investigate the effect of SrR, CeO, and their combinations on tibia quality in broilers. A total of 384 one-day-old Ross chicks were divided into six treatments, with four replicates per treatment (16 birds per replicate). The control group was fed a standard diet, and other groups were fed SrR at levels 450, 900 mg/kg feed, CeO at levels 300 and 600 mg/kg feed and a combination of 450 SrR + 300 CeO mg/kg feed. Bone mineral density (BMD), bone mineral content (BMC), bone strength (BS), tibia area, tibia weight, bone Length, bone diameter, minerals in tibia bone of male broilers, alkaline phosphatase gene (ALP) and osteocalcin gene (OC) in male broilers were analysed.

RESULTS

The results showed that the addition of SrR and CeO had no significant influence (p > 0.01) on BMD, BMC, BS, bone weight, bone length and bone diameter. While there was a significant interaction between sex and treatments, especially in the combination group, BS in females significantly (p < 0.01) increased compared to the control group. Generally, females were found to be more responsive to treatments than males. Significant increases in gene expression were noticed in OC with the addition of low levels of SrR and CeO and mixed group compared to the control. The gene expression of ALP was increased significantly only in a combination group compared to the control group.

CONCLUSION

It is concluded that SrR and CeO can be used as beneficial additives in the feed to improve the tibia quality of broilers.

摘要

背景

在现代肉鸡产业中，腿部和步态异常是一个严重的问题。快速生长的肉鸡尤其容易出现骨骼异常，这给肉鸡生产者带来了重大问题。雷奈酸锶（SrR）已成功用于治疗人类骨质疏松症。此外，氧化铈（CeO）是一种应用于生物系统的抗应激剂。

方法

本研究旨在探讨 SrR、CeO 及其组合对肉鸡胫骨质量的影响。将 384 只 1 日龄罗斯小鸡分为 6 个处理组，每个处理组设 4 个重复（每个重复 16 只鸡）。对照组饲喂基础日粮，其他组分别在基础日粮中添加 450、900mg/kg 饲料水平的 SrR、300、600mg/kg 饲料水平的 CeO 以及 450SrR+300CeOmg/kg 饲料的混合物。分析了雄性肉鸡的骨密度（BMD）、骨矿物质含量（BMC）、骨强度（BS）、胫骨面积、胫骨重量、骨长、骨直径、胫骨骨矿物质含量、碱性磷酸酶基因（ALP）和骨钙素基因（OC）。

结果

结果表明，添加 SrR 和 CeO 对 BMD、BMC、BS、骨重、骨长和骨直径没有显著影响（p＞0.01）。然而，性别和处理之间存在显著的相互作用，尤其是在组合组中，与对照组相比，雌性 BS 显著增加（p＜0.01）。一般来说，雌性对处理的反应比雄性更敏感。与对照组相比，添加低水平 SrR 和 CeO 以及混合组可显著增加 OC 基因的表达。与对照组相比，仅在组合组中 ALP 基因的表达显著增加。