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12 岁以下患者口腔 的诊断: 作为 分子特征分析的标志物。

Diagnosis of Oral in Patients under 12 Years: as a Marker of Molecular Characterization of .

机构信息

College of Pharmacy, Al-Ayen University, Thi-Qar, 64001, Iraq.

Department of Biology, College of Education for Pure Science, Thi-Qar University, Thi-Qar 64001, Iraq.

出版信息

Arch Razi Inst. 2023 Feb 28;78(1):475-483. doi: 10.22092/ARI.2022.359466.2422. eCollection 2023 Feb.

Abstract

has been found as the most abundant pathogenic yeast species under the group Candida-non-albicans. Despite this, it is taxonomically related to and has many of its pathogenic characteristics. Infection with is closely associated with many virulence factors encoded by multiple virulence genes. This study aims to diagnose based on the presence of and to detect many virulence genes. isolates were collected from oral candidiasis patients. Children infected with oral thrush ranging in age from infants to 12 years old provided 150 samples. (66.68 %), (13.21 %), (9.43 %), (7.55 %), and were isolated as types, according to the findings of the present study (2.83%). The presence of the gene was confirmed in the isolates. All isolates were positive for and , while some were positive for (78.5%) and genes (71.4%). Using sequences and phylogenetic trees, it was determined that there was negligible genetic variation between local isolates and global strains. These virulence factor genes play a crucial role in developing infections.

摘要

被发现是最丰富的致病性酵母物种组念珠菌属非白念珠菌。尽管如此,它在分类上与密切相关,并具有许多致病性特征。感染与许多毒力因子密切相关,这些毒力因子由多个毒力基因编码。本研究旨在基于的存在来诊断,并检测许多毒力基因。从口腔念珠菌病患者中采集了 分离株。年龄在婴儿到 12 岁之间的儿童感染鹅口疮,提供了 150 个样本。根据本研究的发现,被鉴定为 (66.68%)、 (13.21%)、 (9.43%)、 (7.55%)和 (2.83%)。在分离株中确认了 基因的存在。所有分离株均为 和 阳性,而有些分离株为 和 基因阳性(78.5%)和 基因阳性(71.4%)。使用序列和系统发育树,确定本地分离株与全球菌株之间遗传变异很小。这些毒力因子基因在感染的发展中起着至关重要的作用。

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