Department of Clinical Pharmacology, Faculty of Medicine, Al-Azhar University, Damietta, Egypt.
Department of Clinical Pharmacology, Faculty of Medicine, Helwan University, Cairo, Egypt.
Arch Razi Inst. 2023 Feb 28;78(1):151-159. doi: 10.22092/ARI.2022.358340.2202. eCollection 2023 Feb.
Both animal and human studies have documented cognitive and behavioural impairment after exposure to inhalational anaesthetics. Therefore, the current study was designed to demonstrate if the anaesthetics isoflurane and Sevoflurane can result in postoperative cognition dysfunction in normal and diabetic rats. Sixty male Wister rats aged 12 weeks were divided into 6 groups (n=10); group C (standard control), group CD (diabetic control), group S (sevoflurane anaesthesia), group I (isoflurane anaesthesia), group SD (diabetic sevoflurane anaesthesia) group ID (diabetic isoflurane anaesthesia). Animals were anaesthetized with either 2. 5% sevoflurane or 1.5% isoflurane, respectively, for 2h. 1 week later, animals were undergone cognitive tests in (a Morris water maze, T maze and open field arena), the animals were sacrificed, and hippocampus homogenates were studied for caspase 3 activity by western blot assay. Induction of type II diabetes in CD, SD and ID groups was carried out by feeding on a high-fat diet for 8 weeks before the start of the experiment. During the fourth week, Type II diabetes was induced in the experimental group by a single IP injection of 30 mg/kg STZ. Control (normal and diabetic) rats showed no change in long-term/reference memory, non-spatial working memory, exploratory activity or caspase 3 expression in the hippocampus homogenate. Anaesthesia with isoflurane in normoglycemic rats resulted in a significant decline in long-term/reference memory and non-spatial working memory, while exploratory activity and caspase 3 expressions in hippocampus homogenate showed no change to normal control rats. Both isoflurane and Sevoflurane in diabetic rats demonstrated a decline in long-term/reference memory, non-spatial working memory, exploratory activity and caspase 3 expression in hippocampus homogenate compared with normal control rats. Diabetes revealed significant post-anaesthesia cognitive dysfunction after anaesthesia with Sevoflurane or isoflurane in all the studied domains compared to standard control or diabetic control.
动物和人体研究都记录了吸入麻醉剂暴露后认知和行为障碍。因此，本研究旨在证明麻醉剂异氟烷和七氟烷是否会导致正常和糖尿病大鼠术后认知功能障碍。60 只 12 周龄雄性 Wistar 大鼠分为 6 组（n=10）；组 C（标准对照）、组 CD（糖尿病对照）、组 S（七氟烷麻醉）、组 I（异氟烷麻醉）、组 SD（糖尿病七氟烷麻醉）、组 ID（糖尿病异氟烷麻醉）。动物分别用 2.5%七氟烷或 1.5%异氟烷麻醉 2h。1 周后，动物在（a Morris 水迷宫、T 迷宫和开放场竞技场）进行认知测试，动物被处死，海马匀浆通过 Western blot 检测 caspase 3 活性。CD、SD 和 ID 组的 2 型糖尿病通过在实验前 8 周喂养高脂肪饮食诱导。在第四周，实验组通过单次腹腔注射 30mg/kg STZ 诱导 2 型糖尿病。对照（正常和糖尿病）大鼠在长期/参考记忆、非空间工作记忆、探索性活动或海马匀浆中 caspase 3 表达方面没有变化。正常血糖大鼠异氟烷麻醉导致长期/参考记忆和非空间工作记忆显著下降，而海马匀浆中探索性活动和 caspase 3 表达与正常对照组大鼠无变化。糖尿病大鼠异氟烷和七氟烷麻醉后，与正常对照组大鼠相比，长期/参考记忆、非空间工作记忆、海马匀浆中探索性活动和 caspase 3 表达均下降。与标准对照或糖尿病对照相比，七氟烷或异氟烷麻醉后，糖尿病大鼠在所有研究领域均表现出显著的麻醉后认知功能障碍。
