Li Yingshuo, Sun Minghao, Sun Yueqi, Wang Mingqiang, Zhao Fuwei
Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing, China.
Forestry College, Nanjing Forestry University, Nanjing, China.
Mitochondrial DNA B Resour. 2023 Jun 9;8(6):639-642. doi: 10.1080/23802359.2023.2220433. eCollection 2023.
var. is a valuable multiuse tree with a long history of use in China. It has great economic and ecological importance and is used for landscape and urban planting, soil improvement, and raw materials for food production. However, genomic studies of var. are limited. Meanwhile, the classification of this taxon is controversial. In this study, the complete plastome of var. was successfully sequenced and assembled. The chloroplast genome is 157,215 bp in length with a 37.3% GC content. The chloroplast genome structure includes a quadripartite structure comprising a pair of inverted repeat (IR) sequences of 25,872 bp, a small single-copy (SSC) region of 18,216 bp, and a large single-copy (LSC) region of 87,255 bp. The genome contains 128 genes, including 83 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic inference based on complete plastome analysis showed that var. is closely related to and belongs to the family Pentaphylacaceae, which differs from the results of the traditional Engler system. The chloroplast genome sequence assembly and phylogenetic analysis enrich the genetic resources of Pentaphylacaceae and provide a molecular basis for further studies on the phylogeny of the family.
变种是一种具有重要多种用途的树木，在中国有着悠久的使用历史。它具有重大的经济和生态意义，用于景观和城市绿化、土壤改良以及食品生产原料。然而，该变种的基因组研究有限。同时，这个分类单元的分类存在争议。在本研究中，成功测序并组装了该变种的完整质体基因组。叶绿体基因组长度为157,215 bp，GC含量为37.3%。叶绿体基因组结构包括四分体结构，由一对25,872 bp的反向重复（IR）序列、一个18,216 bp的小单拷贝（SSC）区域和一个87,255 bp的大单拷贝（LSC）区域组成。该基因组包含128个基因，包括83个蛋白质编码基因、37个tRNA基因和8个rRNA基因。基于完整质体基因组分析的系统发育推断表明，该变种与[某物种]密切相关，属于五列木科，这与传统恩格勒系统的结果不同。叶绿体基因组序列组装和系统发育分析丰富了五列木科的遗传资源，并为该科系统发育的进一步研究提供了分子基础。
