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一种野生烟草叶绿体全基因组的组装及系统发育分析

Assembly and phylogenetic analysis of complete chloroplast genome of a wild tobacco .

作者信息

Lin Haifeng, Bai Di

机构信息

College of Information Science and Technology, Nanjing Forestry University, Nanjing, Jiangsu, China.

College of Engineering, Nanjing Agricultural University, Nanjing, Jiangsu, China.

出版信息

Mitochondrial DNA B Resour. 2017 Nov 6;2(2):778-780. doi: 10.1080/23802359.2017.1398600.

Abstract

() is an annual herb that induces nicotine, volatiles and phenolics in response to damage. In this study, we assembled the complete chloroplast (cp) genome of with the length of 155,886 bp, which was composed of a large single copy region (LSC) of 86,602 bp, a small copy (SSC) region of 18,518 bp and a pair of inverted repeats (IR) of 25,383 bp. This typical cp genome contains 112 unique genes, including 78 protein-coding genes, 30 tRNA genes and four rRNA genes. Phylogenetic analysis of 32 plant cp genomes based on 76 conserved protein-coding genes shows that is evolutionarily close to , then . The complete cp genome sequence of may provide a crucial foundation for the study of evolution, molecular biology and genetic engineering in species.

摘要

(某植物名称)是一种一年生草本植物,受到损伤时会产生尼古丁、挥发性物质和酚类物质。在本研究中,我们组装了(某植物名称)的完整叶绿体(cp)基因组,其长度为155,886 bp,由一个86,602 bp的大单拷贝区域(LSC)、一个18,518 bp的小单拷贝(SSC)区域和一对25,383 bp的反向重复序列(IR)组成。这个典型的cp基因组包含112个独特基因,包括78个蛋白质编码基因、30个tRNA基因和4个rRNA基因。基于76个保守蛋白质编码基因对32个植物cp基因组进行的系统发育分析表明,(某植物名称)在进化上与(另一植物名称)接近,其次是(又一植物名称)。(某植物名称)的完整cp基因组序列可能为该物种的进化、分子生物学和基因工程研究提供关键基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c57/7800318/7e6fb47623a0/TMDN_A_1398600_F0001_B.jpg

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