Pan Tingting, He Mingliang, Liu Hualong, Tian Xiaojie, Wang Zhenyu, Yu Xinglong, Miao Xingfen, Li Xiufeng
College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, 163319 China.
Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081 China.
Mol Breed. 2022 Oct 6;42(10):63. doi: 10.1007/s11032-022-01334-4. eCollection 2022 Oct.
Flowering time is one of the most fundamental factors that determine the distribution and final yield of rice. Ehd1 (Early heading date 1) is a B-type response regulator which functions as a flowering time activator. Although diverse flowering time genes have been reported as regulatory factors of expression, the potential regulators of largely remain to be identified. Here, we identified a basic leucine zipper transcription factor bZIP65, a homolog of bZIP71, as a new negative regulator of . The overexpression of delays flowering, while mutants have similar flowering time to SJ2 (Songjing2) in both long-day and short-day conditions. Biochemically, bZIP65 associates with promoter and transcriptionally represses the expression of . Moreover, we found that bZIP65 enhances H3K27me3 level of . Taken together, we cloned a new gene, , regulating rice heading date, and uncovered the mechanism of bZIP65 delaying flowering time, where bZIP65 increases the H3K27me3 level of and transcriptionally represses the expression of , similar to its homolog bZIP71.
The online version contains supplementary material available at 10.1007/s11032-022-01334-4.
抽穗期是决定水稻分布和最终产量的最基本因素之一。Ehd1（早抽穗期1）是一种B型响应调节因子，作为抽穗期激活因子发挥作用。尽管已经报道了多种抽穗期基因作为Ehd1表达的调控因子，但Ehd1的潜在调控因子在很大程度上仍有待确定。在这里，我们鉴定了一种基本亮氨酸拉链转录因子bZIP65，它是bZIP71的同源物，作为Ehd1的一种新的负调控因子。bZIP65的过表达延迟开花，而bZIP65突变体在长日照和短日照条件下的抽穗期与SJ2（松粳2号）相似。在生化方面，bZIP65与Ehd1启动子结合并转录抑制Ehd1的表达。此外，我们发现bZIP65提高了Ehd1的H3K27me3水平。综上所述，我们克隆了一个调控水稻抽穗期的新基因Ehd1，并揭示了bZIP65延迟开花时间的机制，其中bZIP65增加了Ehd1的H3K27me3水平并转录抑制Ehd1的表达，类似于其同源物bZIP71。
在线版本包含可在10.1007/s11032-022-0
