Lu Xin, Torabi Sepideh, de Lima Passianotto André Luiz, Welacky Tom, Eskandari Milad
Department of Plant Agriculture, University of Guelph, Guelph, Canada.
Agriculturan and Agri-Food Canada, Harrow Research Station, Essex, Canada.
Mol Breed. 2022 Sep 29;42(10):62. doi: 10.1007/s11032-022-01330-8. eCollection 2022 Oct.
Soybean cyst nematode (SCN) is one of the most damaging soybean () pests worldwide. More than 95% of SCN-resistant commercial cultivars in North America are derived from a single source of resistance named PI 88788, and the widespread use of this source in the past three decades has led to the selection of virulent biotypes of SCN, such as HG () type 2.5.7 that can overcome the PI 88788-type resistance. The objectives of this study were to identify quantitative trait loci (QTL) and candidate genes underlying the resistance to HG type 2.5.7 isolate and to measure the impact of the resistance factors on seed yield. To achieve the goals, a recombinant inbreed line (RIL) population was established from a cross between an SCN-susceptible high-yielding elite soybean cultivar, OAC Calypso, and the cultivar LD07-3419, resistant to SCN HG type 2.5.7. The RILs resistant to HG type 2.5.7 were identified using greenhouse bioassay tested for differentiation of resistant sources using Kompetitive Allele-Specific PCR (KASP) assay at and loci and also for copy number variation using TaqMan assay. The RILs were also genotyped using genotype-by-sequencing and three SCN-related QTL were identified on chromosomes 9, 12, and 18 using composite interval mapping. In addition, 31 genes involved in protein kinase activity were identified within QTL regions as potential causal candidate genes underlying the resistance. No significant correlation was found between seed yield and the resistance to SCN in the RIL population evaluated under non-SCN-infested environments.
The online version contains supplementary material available at 10.1007/s11032-022-01330-8.
大豆胞囊线虫（SCN）是全球最具破坏性的大豆害虫之一。北美超过95%的抗SCN商业品种都源自单一抗性来源PI 88788，在过去三十年中该抗性来源的广泛使用导致了SCN毒性生物型的出现，比如能克服PI 88788型抗性的HG（）2.5.7型。本研究的目的是鉴定对HG 2.5.7分离株抗性的数量性状位点（QTL）和候选基因，并衡量抗性因子对种子产量的影响。为实现这些目标，从一个对SCN敏感的高产优良大豆品种OAC Calypso与对SCN HG 2.5.7有抗性的品种LD07 - 3419杂交后代中构建了一个重组自交系（RIL）群体。通过温室生物测定法鉴定对HG 2.5.7型有抗性的RIL，利用竞争性等位基因特异性PCR（KASP）测定法在和位点测试抗性来源的分化情况，并利用TaqMan测定法检测拷贝数变异。还通过简化基因组测序对RIL进行基因分型，并使用复合区间作图法在第9、12和18号染色体上鉴定出3个与SCN相关的QTL。此外，在QTL区域内鉴定出31个参与蛋白激酶活性的基因作为抗性潜在的因果候选基因。在未受SCN侵染的环境中评估的RIL群体中，未发现种子产量与对SCN的抗性之间存在显著相关性。
在线版本包含可在10.1007/s11032 - 022 - 01330 - 8获取的补充材料。
