番茄对[具体病害名称未给出]抗性的KASP标记的开发与验证
Development and validation of KASP markers for resistance to in L.
作者信息
Zhang Zhenghai, Cao Yacong, Wang Yongfu, Yu Hailong, Wu Huamao, Liu Jing, An Dongliang, Zhu Yanshu, Feng Xigang, Zhang Baoxi, Wang Lihao
机构信息
Key Laboratory of Vegetable Genetics and Physiology of the Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Beijing, 100081 China.
出版信息
Mol Breed. 2023 Mar 11;43(3):20. doi: 10.1007/s11032-023-01367-3. eCollection 2023 Mar.
UNLABELLED
Resistance of to blight is dependent on the genetic background of the resistance source and the isolate, which poses challenges for development of generally applicable molecular markers for marker-assisted selection. In this study, the resistance to of was genetically mapped to chromosome 5 within a 1.68-Mb interval by genome-wide association study analysis of 237 accessions. In this candidate region, 30 KASP markers were developed using genome resequencing data for a -resistant line (0601 M) and a susceptible line (77,013). Seven of these KASP markers, located in the coding region of a probable leucine-rich repeats receptor-like serine/threonine-protein kinase gene (), were validated in the 237 accessions, which showed an average accuracy of 82.7%. The genotyping of the seven KASP markers strongly corresponded with the phenotype of 42 individual plants in a pedigree family (PC83-163) developed from the -resistant line CM334. This research provides a set of efficient and high-throughput KASP markers for marker-assisted selection of resistance to in .
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s11032-023-01367-3.
未标记
对疫病的抗性取决于抗性来源的遗传背景和疫病分离株，这给开发适用于标记辅助选择的通用分子标记带来了挑战。在本研究中，通过对237份种质进行全基因组关联研究分析，将辣椒对疫病的抗性基因定位到5号染色体上一个1.68 Mb的区间内。在这个候选区域，利用一个抗疫病品系（0601 M）和一个感病品系（77,013）的基因组重测序数据开发了30个KASP标记。其中7个KASP标记位于一个可能的富含亮氨酸重复序列的类受体丝氨酸/苏氨酸蛋白激酶基因（）的编码区，在237份种质中得到验证，平均准确率为82.7%。这7个KASP标记的基因分型与从抗疫病品系CM334培育的一个系谱家族（PC83 - 163）中42株单株的表型高度一致。本研究为辣椒疫病抗性的标记辅助选择提供了一套高效、高通量的KASP标记。
补充信息
在线版本包含可在10.1007/s11032 - 023 - 01367 - 3获取的补充材料。
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