Development and Application of a Cleaved Amplified Polymorphic Sequence Marker () Linked to the Locus Conferring Resistance to in Pepper ( L.).

causes destructive disease in several crop species, including pepper ( L.). Resistance in this species is physiologically and genetically complex due to many virulence phenotypes and different QTLs and R genes among the identified resistance sources. Several primer pairs were designed to follow an SNP (G/A) within the locus linked to the locus. All primer pairs were designed on DNA sequences derived from , a homoserine kinase (HSK), which is a gene candidate responsible for the major QTL on chromosome for resistance to . A panel of 69 pepper genotypes from the Southern Seed germplasm collection was used to screen the primer pairs designed. Of these, two primers (_for_2 and _rev_2) surrounding the SNP proved successful in discriminating susceptible and resistant genotypes when combined with a restriction enzyme (). This new marker (called ) worked as expected in all genotypes tested, proving to be an excellent candidate for marker-assisted selection in breeding programs aimed at introgressing the resistant locus into pure lines.