Xu Zuopeng, Li Meng, Du Yuanyue, Li Xixu, Wang Ruixuan, Chen Zhiai, Tang Shuzhu, Liu Qiaoquan, Zhang Honggen
Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Agricultural College of Yangzhou University, Yangzhou, 225009 China.
Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009 China.
Mol Breed. 2022 Nov 3;42(11):70. doi: 10.1007/s11032-022-01339-z. eCollection 2022 Nov.
Panicle length (PL) is an important trait that determines panicle architecture and strongly affects grain yield and quality in rice. However, this trait has not been well characterized genetically, and its contribution to yield improvement is not well understood. Characterization of novel genes related to PL is of great significance for breeding high-yielding rice varieties. In our previous research, we identified , a quantitative trait locus for PL. In this study, we aimed to determine the exact position of in the rice genome and identify the candidate gene. Through substitution mapping, we mapped to a region of 21.86 kb flanked by the molecular marker loci STS5-99 and STS5-106 in which two candidate genes were predicted. By sequence analysis and relative expression analysis, , which putatively encodes a BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 precursor, was considered to be the most likely candidate gene for . In addition, we successfully developed a pair of near-isogenic lines (NILs) for in different genetic backgrounds to evaluate the genetic effects of . Agronomic trait analysis of the NILs indicated that positively contributes to plant height, grain number per panicle, panicle length, grain yield per plant, and flag leaf length, but it had no influence on heading date and grain-size-related traits. Therefore, and the markers tightly linked to it should be available for molecular breeding of high-yielding varieties.
The online version contains supplementary material available at 10.1007/s11032-022-01339-z.
穗长（PL）是决定穗型结构的重要性状，对水稻产量和品质有重大影响。然而，该性状的遗传特征尚未得到充分表征，其对产量提高的贡献也未被充分理解。鉴定与穗长相关的新基因对培育高产水稻品种具有重要意义。在我们之前的研究中，我们鉴定出一个穗长的数量性状位点。在本研究中，我们旨在确定该位点在水稻基因组中的精确位置并鉴定候选基因。通过代换作图，我们将该位点定位到分子标记位点STS5 - 99和STS5 - 106侧翼的21.86 kb区域，该区域预测有两个候选基因。通过序列分析和相对表达分析，推测编码一种油菜素内酯不敏感1相关受体激酶1前体的基因被认为是最有可能的候选基因。此外，我们成功地在不同遗传背景下开发了一对该位点的近等基因系（NILs），以评估该位点的遗传效应。对这些近等基因系的农艺性状分析表明，该位点对株高、每穗粒数、穗长、单株产量和剑叶长度有正向贡献，但对抽穗期和粒型相关性状没有影响。因此，该位点及其紧密连锁的标记可用于高产水稻品种的分子育种。
在线版本包含可在10.1007/s11032 - 022 - 01339 - z获取的补充材料。
