Tamari Y, Ohmori S, Hiraki K
Clin Chem. 1986 Aug;32(8):1464-7.
For fluorometry of selenium in human blood, hair, and liver and in leaves, we wet-ashed the samples with conventional nitric and perchloric acids, and then extracted piazselenol (complex of Se and 2,3-diaminonaphthalene) in cyclohexane. Selenium was back-extracted from the cyclohexane into nitric acid to remove the fluorometric interferences of trace amounts of organic compounds. This fluorometric method is rapid and suitable for routine analysis. We applied the method to human hair samples and compared it with the data for non-destructive neutron activation analysis of the hair.
对于人体血液、毛发、肝脏以及树叶中硒的荧光测定，我们用常规的硝酸和高氯酸对样品进行湿灰化处理，然后在环己烷中萃取piazselenol（硒与2,3 - 二氨基萘的络合物）。将硒从环己烷中反萃取到硝酸中，以消除痕量有机化合物的荧光干扰。这种荧光测定方法快速且适用于常规分析。我们将该方法应用于人体毛发样品，并与毛发的无损中子活化分析数据进行了比较。
