Chen S Y, Collipp P J, Boasi L H, Isenschmid D S, Verolla R J, San Roman G A, Yeh J K
Ann Nutr Metab. 1982;26(3):186-90. doi: 10.1159/000176562.
This communication introduces a special lyophilization process for selenium determination by fluorometric methods. It permits a small sample volume, with several modifications including a single test tube process. Samples and standard are lyophilized first, then digested with nitric-perchloric acid mixture in a heated sand bath. Selenium is complexed with 2,3-diaminonaphthalene and extracted by n-hexane in the same test tube. The n-hexane layer is transferred to a cuvette and measured fluorometrically. Selenium concentration in healthy children from Long Island (aged 5-18 years) was: hair 0.765 +/- 0.114 microgram/g (n = 52), urine 28.65 +/- 8.27 micrograms/g creatinine (n = 66), and serum 95.4 +/- 14.4 ng/ml (n = 44). The current literature reflects an increase in the role of selenium in human nutrition. Thus, a simple but reliable method for determination of selenium in biological materials is needed in the clinical and research laboratory.
本通讯介绍了一种用于荧光法测定硒的特殊冻干方法。它允许使用少量样品，并进行了若干改进，包括采用单试管法。首先将样品和标准品冻干，然后在加热的沙浴中用硝酸 - 高氯酸混合物消化。硒与2,3 - 二氨基萘络合，并在同一试管中用正己烷萃取。将正己烷层转移至比色皿中并进行荧光测定。长岛健康儿童（5 - 18岁）的硒浓度为：头发0.765±0.114微克/克（n = 52），尿液28.65±8.27微克/克肌酐（n = 66），血清95.4±14.4纳克/毫升（n = 44）。当前文献反映出硒在人类营养中的作用有所增加。因此，临床和研究实验室需要一种简单而可靠的生物材料中硒的测定方法。
