1Department of Virology, Faculty of Veterinary Medicine, Balikesir University, 10100, Balıkesir, Türkiye.
2Department of Pathology, Faculty of Veterinary Medicine, Balikesir University, 10100, Balıkesir, Türkiye.
Acta Vet Hung. 2023 Jun 13;71(1):54-64. doi: 10.1556/004.2023.00809. Print 2023 Jun 20.
Canine distemper virus (CDV) is a highly contagious virus that infects a wide variety of animals of carnivore species and may cause manifestations from subclinical infection to fatal disease. In this study, dogs clinically suspected having distemper were examined by reverse transcriptase-polymerase chain reaction (RT-PCR), histopathology and immuno-histochemistry. By histopathological examination, characteristic intracytoplasmic and/or intranuclear inclusion bodies were observed in the lung, stomach, small intestine, liver, kidney, spleen and central nervous system. Interstitial and broncho-interstitial pneumonia, gastroenteritis and encephalitis were revealed. CDV antigens were detected in all tissues with characteristic histopathological findings. The antigens were more abundant in the bronchial and bronchiolar epithelium and in the syntitial cells. Phylogenetic analyses were performed using the PCR-amplified partial sequences of the genes encoding the viral heamagglutinin and fusion proteins. The phylogenetic trees showed that the newly determined sequences were diverse and clustered within different lineages of the European or the Arctic strains.
犬瘟热病毒（CDV）是一种高度传染性病毒，可感染多种肉食动物物种，并可能导致从亚临床感染到致命疾病的表现。在这项研究中，通过逆转录-聚合酶链反应（RT-PCR）、组织病理学和免疫组织化学检查，对临床怀疑患有犬瘟热的犬进行了检查。通过组织病理学检查，在肺、胃、小肠、肝、肾、脾和中枢神经系统中观察到特征性的细胞质和/或核内包涵体。揭示了间质性和细支气管炎性肺炎、胃肠炎和脑炎。在具有特征性组织病理学发现的所有组织中均检测到 CDV 抗原。在支气管和细支气管上皮细胞以及基质细胞中，抗原更为丰富。使用编码病毒血凝素和融合蛋白的基因的 PCR 扩增部分序列进行了系统发育分析。系统发育树表明，新确定的序列是多样的，并在欧洲或北极株的不同谱系内聚类。
