Immunisation with Transgenic Expressing Gamma Glutamyl Cysteine Synthetase from Pathogenic Species Protected against and Infection in a Murine Model.

Leishmaniasis is a protozoan disease responsible for significant morbidity and mortality. There is no recommended vaccine to protect against infection. In this study, transgenic expressing gamma glutamyl cysteine synthetase (γGCS) from three pathogenic species were produced and their ability to protect against infection determined using models of cutaneous and visceral leishmaniasis. The ability of IL-2-producing PODS to act as an adjuvant was also determined in studies. Two doses of the live vaccine caused a significant reduction in ( < 0.001) and ( < 0.05) parasite burdens compared to their respective controls. In contrast, immunisation with wild type , using the same immunisation protocol, had no effect on parasite burdens compared to infection controls. Joint treatment with IL-2-producing PODS enhanced the protective effect of the live vaccine in studies. Protection was associated with a Th1 response in and a mixed Th1/Th2 response in , based on specific IgG1 and IgG2a antibody and cytokine production from in vitro proliferation assays using antigen-stimulated splenocytes. The results of this study provide further proof that γGCS should be considered a candidate vaccine for leishmaniasis.