Natural Products Division, Forest Research Institute Malaysia, Kepong, Selangor, Malaysia.
School of Health Sciences, University Sains Malaysia, Kelantan, Malaysia.
Ann Med. 2023 Dec;55(1):2224970. doi: 10.1080/07853890.2023.2224970.
(L.) Urb. (Apiaceae) is a renowned medicinal plant being used in the Ayurvedic system for its pharmacological effects on the central nervous system such as rejuvenating, sedative, anxiolytic and memory-enhancing properties. The present study was designed to investigate the effect of (CA) extract on inflammatory responses induced by lipopolysaccharide (LPS) and resulting changes in cognitive behavior.
Adult male Sprague-Dawley rats were divided into 4 groups as control, LPS, CA and LPS + CA. The treatments with LPS (5 mg/kg) were intraperitoneally (i.p) injected on day 4 and CA ethanol extract (200 mg/kg) were given orally for 14 days. Morris Water Maze (MWM) test was performed to assess spatial learning and memory performance. Acute oral toxicity of the extract at the highest dose of 5000 mg/kg was also conducted.
Single administration of LPS was able to significantly elicit learning and memory impairment ( < .05) when compared to the control groups. Treatment with CA significantly improved the impaired learning ability in which the LPS + CA rats took the shortest time and route to find the hidden platform (15.85 ± 2.68 s ( < .001); 352.43 ± 88.10 cm ( < .001) on day 5) and induced differential cytokine responses in the blood. No mortality and no significant variation in the body and organ weights between the control and the treated group was observed after 14 days of acute toxicity study. Hematological analysis and biochemical parameters revealed no toxic effects of the extract. Pathologically, neither gross abnormalities nor histopathological changes were observed.
extract exhibited significant learning and memory enhancement potential in animal model. Hence, indicating its putative preventive therapeutic effects in neuroinflammation related diseases.KEY MESSAGEA single dose of lipopolysaccharide (LPS) (5 mg/kg) administered systemically to mimic the consequences of LPS-induced inflammatory responses was able to affect some behavioral modification of spatial memory at the time point of study.The study showed that the learning capability during the training trial was restored or ameliorated with the pre-emptive treatment of extract (200 mg/kg). extract improves spatial memory, learning deficits and regulates proinflammatory responses in systemic LPS-treated rats.
（L.）Urb.（伞形科）是一种著名的药用植物，在阿育吠陀体系中因其对中枢神经系统的药理学作用而被使用，如 rejuvenating、sedative、anxiolytic 和 memory-enhancing 特性。本研究旨在研究（CA）提取物对脂多糖（LPS）诱导的炎症反应的影响以及由此导致的认知行为变化。
成年雄性 Sprague-Dawley 大鼠分为 4 组：对照组、LPS 组、CA 组和 LPS+CA 组。LPS（5mg/kg）的处理于第 4 天经腹腔（i.p）注射，CA 乙醇提取物（200mg/kg）连续 14 天口服给予。Morris 水迷宫（MWM）测试用于评估空间学习和记忆表现。还进行了提取物在最高剂量 5000mg/kg 时的急性口服毒性。
单次 LPS 给药与对照组相比，能够显著引起学习和记忆障碍（<0.05）。CA 治疗显著改善了受损的学习能力，其中 LPS+CA 大鼠找到隐藏平台的时间最短，路线最短，分别为 15.85±2.68s（<0.001）和 352.43±88.10cm（<0.001），在第 5 天），并诱导血液中差异的细胞因子反应。在急性毒性研究的 14 天后，在对照组和治疗组之间未观察到死亡率和体重及器官重量的显著变化。血液学分析和生化参数显示提取物无毒性作用。病理学上，既没有明显的大体异常，也没有组织病理学变化。
提取物在动物模型中表现出显著的学习和记忆增强潜力。因此，提示其在神经炎症相关疾病中具有潜在的预防治疗作用。
关键信息
单次剂量的脂多糖（LPS）（5mg/kg）全身给药模拟 LPS 诱导的炎症反应的后果，能够影响研究时点时空间记忆的某些行为改变。
研究表明，在训练试验中，学习能力得到恢复或改善，预先用（CA）提取物（200mg/kg）治疗。提取物改善 LPS 处理大鼠的空间记忆、学习缺陷和调节促炎反应。
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