Yadav Mukesh Kumar, Singh Santosh Kumar, Singh Manish, Mishra Shashank Shekhar, Singh Anurag Kumar, Tripathi Jyoti Shankar, Tripathi Yamini Bhusan
Department of Kayachikitsa, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
Centre of Experimental Medicine & Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
Open Access Maced J Med Sci. 2019 Apr 11;7(7):1071-1076. doi: 10.3889/oamjms.2019.209. eCollection 2019 Apr 15.
We aimed to investigate several parameters after the acute and sub-acute administration of ethanolic extracts from & .
Malignant Ovarian Germ Cell Tumors for toxicity study guidelines 423 and 407 of Organization for Economic Co-operation and Development (OECD) were followed for acute and sub-acute toxicity assays respectively. For LD50 evaluation, a single dose of ethanolic extracts of Evolvulus alsinoides L. (EEA) and ethanolic extracts of Centella asiatica (ECA) was orally administered to mice at doses of 200, 400, 800, 1600 and 2000 mg/kg. Then the animals were observed for 72 hours. For acute toxicity evaluation, a single dose of both extracts was orally administered to mice at doses of 300, 600, 1200 and 2000 mg/kg and the animals were observed for 14 days. In the sub-acute study, the extracts were orally administered to mice for 28 days at doses of 300, 600, 1200 and 2000 mg/kg. To assess the toxicological effects, animals were closely observed on general behaviour, clinical signs of toxicity, body weight, food and water intake. At the end of the study, it was performed biochemical and hematological evaluations, as well as histopathological analysis from the following organs: brain, heart, liver, and kidney.
The oral administration of E. alsinoides and C. asiatica ethanolic extracts, i.e. EEA 300, EEA 600, EEA 1200, EEA 2000, ECA 300, ECA 600, ECA 1200 & ECA 2000 mg/kg doses showed no moral toxicity effect in LD50, acute and sub-acute toxicity parameters.
In this study, we had found that & extract at different doses cause no mortality in acute and sub-acute toxicity study. Also, histopathology of kidney, liver, heart, and brain showed no alterations in tissues morphology.
我们旨在研究从[具体植物1]和[具体植物2]中提取的乙醇提取物在急性和亚急性给药后的几个参数。
分别按照经济合作与发展组织（OECD）的毒性研究指南423和407对恶性卵巢生殖细胞肿瘤进行急性和亚急性毒性试验。为评估半数致死剂量（LD50），将单剂量的阿尔西诺伊旋花乙醇提取物（EEA）和积雪草乙醇提取物（ECA）以200、400、800、1600和2000mg/kg的剂量口服给予小鼠。然后对动物观察72小时。为进行急性毒性评估，将两种提取物的单剂量以300、600、1200和2000mg/kg的剂量口服给予小鼠，并对动物观察14天。在亚急性研究中，将提取物以300、600、1200和2000mg/kg的剂量口服给予小鼠28天。为评估毒理学效应，密切观察动物的一般行为、毒性临床体征、体重、食物和水摄入量。在研究结束时，进行生化和血液学评估，以及对以下器官进行组织病理学分析：脑、心脏、肝脏和肾脏。
口服阿尔西诺伊旋花和积雪草乙醇提取物，即EEA 300、EEA 600、EEA 1200、EEA 2000、ECA 300、ECA 600、ECA 1200和ECA 2000mg/kg剂量，在LD50、急性和亚急性毒性参数方面均未显示出致死毒性效应。
在本研究中，我们发现不同剂量的[具体植物1]和[具体植物2]提取物在急性和亚急性毒性研究中未导致死亡。此外，肾脏、肝脏、心脏和脑的组织病理学显示组织形态无改变。
