College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.
College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China; Third Hospital of Shanxi Medical University (Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital), Taiyuan, 030032, China.
Exp Eye Res. 2023 Aug;233:109541. doi: 10.1016/j.exer.2023.109541. Epub 2023 Jun 13.
Ocular diseases and treatment related to rhegmatogenous retinal detachment (RRD) are highly correlated with retinal adhesion behavior. Therefore, this paper proposes to study the adhesion behavior of the intact retina. This can provide theoretical guidance for the treatment and research of retinal detachment (RD) related diseases. To systematically analyze this aspect, two experiments were performed on the porcine retina. The pull-off test combined with the modified JKR theory was used to study the adhesion behavior of the vitreoretinal interface, while the peeling test was used to study the adhesion behavior of the chorioretinal interface. In addition, the adhesion phase involved in the pull-off test was simulated and analyzed by building the corresponding finite element method (FEM). The experimental results of adhesion force on the vitreoretinal interface were obtained by pull-off test with five sizes of rigid punch. The experimental value of the pull-off force F tends to increase gradually with increasing punch radius in the range of 0.5-4 mm. A comparison of the experimental results with the simulation results shows that they are in a well agreement. And there is no statistical difference between the experimental and theoretical values of the pull-off force F. In addition, the values of retinal adhesion work were also obtained by pull-off test. Interestingly, there is a significant scale effect of the retinal work of adhesion. Finally, the peeling test gave a maximum peeling strength T of about 13 mN/mm and a stable peeling strength T of about 11 mN/mm between the retina and the choroid. The pull-off test well shows the process of retinal traction by the diseased vitreous at the beginning of RRD. A comparison of the experimental results with the finite element results verifies the accuracy of the simulation. The peeling test well investigated the adhesion behavior between the retina and the choroid and obtained key biomechanical data (peeling strength, etc.). The combination of the two experiments allows a more systematic study of the whole retina. This research can provide more complete material parameters for finite element modeling of retina-related diseases, and it also can provide the theoretical guidance for individualized design of retinal repair surgery.
眼球疾病和与孔源性视网膜脱离（RRD）相关的治疗与视网膜粘附行为高度相关。因此，本文提出研究完整视网膜的粘附行为。这可以为视网膜脱离（RD）相关疾病的治疗和研究提供理论指导。为了系统地分析这一方面，本文在猪视网膜上进行了两项实验。使用拉脱试验结合改进的 JKR 理论来研究玻璃体视网膜界面的粘附行为，而使用剥离试验来研究脉络膜视网膜界面的粘附行为。此外，通过构建相应的有限元方法（FEM）对拉脱试验中涉及的粘附阶段进行模拟和分析。通过使用五个尺寸的刚性冲头进行拉脱试验获得了玻璃体视网膜界面的粘附力实验结果。在 0.5-4mm 的范围内，拉脱力 F 的实验值随着冲头半径的增加而逐渐增大。实验结果与模拟结果的比较表明，它们非常吻合，并且拉脱力 F 的实验值和理论值之间没有统计学差异。此外，还通过拉脱试验获得了视网膜粘附功的实验值。有趣的是，视网膜粘附功存在明显的尺度效应。最后，剥离试验得到了视网膜和脉络膜之间约 13mN/mm 的最大剥离强度 T 和约 11mN/mm 的稳定剥离强度 T。拉脱试验很好地显示了 RRD 初期病变玻璃体对视网膜的牵引过程。实验结果与有限元结果的比较验证了模拟的准确性。剥离试验很好地研究了视网膜和脉络膜之间的粘附行为，并获得了关键的生物力学数据（剥离强度等）。这两项实验的结合可以更系统地研究整个视网膜。这项研究可以为与视网膜相关的疾病的有限元建模提供更完整的材料参数，也可以为视网膜修复手术的个体化设计提供理论指导。
