Simeonov I
Vet Med Nauki. 1978;15(7):47-52.
Mass prophylactic vaccinations have radically chaugned the clinical syndrome, pathogenesis, and morphological picture of the Newcastle disease: clinically it is manifested with respiratory troubles, and morphologically--with tracheitis. The method for isolating the virus on chick embryos from immune birds have proved undependable: the higher the immunity of birds, the lower the probability of virus isolation. Following the vaccination of birds after the adopted prophylactic programme and at the three-fold vaccination with a lentogenic La Sota strain and four-fold vaccination with a mesogenic Komarov strain precipitating antibodies have been detected in single serum samples only. In the presence of respiratory troubles in immune birds the rise of antiheamagglutination titers and the mass manifestation of precipitating antibodies in the blood serum is said to be an indication of the presence of a field of velogenic virus, while HI and AGPT are suitable methods for the detection of immune carriers of Newcastle disease. These can be used also for the differential diagnosis of the latent forms of infectious bronchitis, infectious laryngotracheitis, the diphtheroid form of pox, and CRD. In order to restrict and do away with the velogenic field strains of the Newcastle disease virus a suggestion is made to kill all birds that have shown respiratory troubles and positive results in virologic and serologic examinations through HI and AGPT.
大规模预防性疫苗接种已从根本上改变了新城疫的临床综合征、发病机制和形态学表现:临床上表现为呼吸道疾病,形态学上表现为气管炎。从免疫禽类的鸡胚中分离病毒的方法已被证明不可靠:禽类免疫力越高,病毒分离的可能性越低。按照所采用的预防方案对禽类进行疫苗接种后,用弱毒La Sota株进行三次接种,用中等毒力的科马罗夫株进行四次接种后,仅在单个血清样本中检测到沉淀抗体。当免疫禽类出现呼吸道疾病时,抗血凝素滴度升高以及血清中大量出现沉淀抗体被认为是强毒病毒存在的迹象,而血凝抑制试验(HI)和琼脂凝胶沉淀试验(AGPT)是检测新城疫免疫携带者的合适方法。这些方法也可用于传染性支气管炎、传染性喉气管炎、痘疹的假膜性形式和慢性呼吸道疾病(CRD)潜伏形式的鉴别诊断。为了限制和消除新城疫病毒的强毒株,建议扑杀所有在HI和AGPT病毒学和血清学检查中出现呼吸道疾病且结果呈阳性的禽类。