Huang Weifeng, Hu Shuqin, Zhu Yongzhe, Liu Shijia, Zhou Xingya, Fang Yuan, Lu Yihan, Wang Ruilan
Department of Intensive Care Medicine, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Critical Care Medicine, Shanghai General Hospital of Nanjing Medical University, Shanghai, China.
Front Microbiol. 2023 May 30;14:1157888. doi: 10.3389/fmicb.2023.1157888. eCollection 2023.
, a strictly intracellular bacterium, is an underestimated etiologic agent leading to infections in a broad range of animals and mild illness or pneumonia in humans. In this study, the metagenomes of bronchoalveolar lavage fluids from the patients with pneumonia were sequenced and highly abundant was found. The target-enriched metagenomic reads were recruited to reconstruct draft genomes with more than 99% completeness. Two strains from novel sequence types were detected and these were closely related to the animal-borne isolates derived from the lineages of ST43 and ST28, indicating the zoonotic transmissions of would benefit its prevalence worldwide. Comparative genomic analysis combined with public isolate genomes revealed that the pan-genome of possessed a more stable gene repertoire than those of other extracellular bacteria, with ~90% of the genes per genome being conserved core genes. Furthermore, the evidence for significantly positive selection was identified in 20 virulence-associated gene products, particularly bacterial membrane-embedded proteins and type three secretion machines, which may play important roles in the pathogen-host interactions. This survey uncovered novel strains of causing pneumonia and the evolutionary analysis characterized prominent gene candidates involved in bacterial adaptation to immune pressures. The metagenomic approach is of significance to the surveillance of difficult-to-culture intracellular pathogens and the research into molecular epidemiology and evolutionary biology of .
[细菌名称]是一种严格的细胞内细菌，是一种被低估的病原体，可导致多种动物感染以及人类的轻度疾病或肺炎。在本研究中，对肺炎患者支气管肺泡灌洗液的宏基因组进行了测序，发现了高丰度的[细菌名称]。通过招募目标富集的宏基因组读数来重建完整性超过99%的基因组草图。检测到来自新序列类型的两株[细菌名称]菌株，它们与源自ST43和ST28谱系的动物源性分离株密切相关，表明[细菌名称]的人畜共患病传播有利于其在全球范围内的流行。比较基因组分析结合公共分离株基因组显示，[细菌名称]的泛基因组比其他细胞外细菌具有更稳定的基因库，每个基因组中约90%的基因是保守的核心基因。此外，在20种毒力相关基因产物中发现了显著正选择的证据，特别是细菌膜嵌入蛋白和三型分泌机器，它们可能在病原体与宿主的相互作用中发挥重要作用。这项调查发现了导致肺炎的[细菌名称]新菌株，进化分析确定了参与细菌适应免疫压力的重要基因候选物。宏基因组方法对于难以培养的细胞内病原体的监测以及[细菌名称]的分子流行病学和进化生物学研究具有重要意义。
