Prostak Sarah M, Medina Edgar M, Kalinka Erik, Fritz-Laylin Lillian K
Department of Biology, University of Massachusetts Amherst, Amherst, MA, USA.
Access Microbiol. 2023 May 11;5(5). doi: 10.1099/acmi.0.000566.v3. eCollection 2023.
Chytrid fungi play key ecological roles in aquatic ecosystems, and some species cause a devastating skin disease in frogs and salamanders. Additionally, chytrids occupy a unique phylogenetic position- sister to the well-studied Dikarya (the group including yeasts, sac fungi, and mushrooms) and related to animals- making chytrids useful for answering important evolutionary questions. Despite their importance, little is known about the basic cell biology of chytrids. A major barrier to understanding chytrid biology has been a lack of genetic tools with which to test molecular hypotheses. Medina and colleagues recently developed a protocol for -mediated transformation of . In this manuscript, we describe the general procedure including planning steps and expected results. We also provide in-depth, step-by-step protocols and video guides for performing the entirety of this transformation procedure on protocols.io (dx.doi.org/10.17504/protocols.io.x54v9dd1pg3e/v1).
壶菌在水生生态系统中发挥着关键的生态作用，一些物种会在青蛙和蝾螈身上引发毁灭性的皮肤病。此外，壶菌占据着独特的系统发育位置——是经过充分研究的双核菌亚界（包括酵母、子囊菌和蘑菇的类群）的姐妹群，且与动物相关——这使得壶菌对于解答重要的进化问题很有用处。尽管它们很重要，但人们对壶菌的基本细胞生物学了解甚少。理解壶菌生物学的一个主要障碍是缺乏用于检验分子假说的遗传工具。梅迪纳及其同事最近开发了一种用于[具体物种]介导转化的方案。在本手稿中，我们描述了一般程序，包括规划步骤和预期结果。我们还在protocols.io（dx.doi.org/10.17504/protocols.io.x54v9dd1pg3e/v1）上提供了关于执行整个转化程序的深入、分步方案和视频指南。
