Chen Qiutong, Li Li, Qi Xiwu, Fang Hailing, Yu Xu, Bai Yang, Chen Zequn, Liu Qun, Liu Dongmei, Liang Chengyuan
Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, Jiangsu, China.
College of Forestry, Nanjing Forestry University, Nanjing, Jiangsu, China.
Front Plant Sci. 2023 May 31;14:1188922. doi: 10.3389/fpls.2023.1188922. eCollection 2023.
L. is an important spice crop and medicinal herb with high economic value. The plant is covered with peltate glandular trichomes, which are responsible for the biosynthesis and secretion of volatile oils. Plant non-specific lipid transfer proteins (nsLTPs) belong to a complex multigenic family involved in various plant physiological processes. Here, we cloned and identified a non-specific lipid transfer protein gene () from , which may positively regulate peltate glandular trichome density and monoterpene metabolism. was expressed in most tissues. The GUS signal driven by the promoter in transgenic was observed in stems, leaves, and roots; it was also expressed in trichomes. was associated with the plasma membrane. Overexpression of in peppermint ( L) significantly increased the peltate glandular trichome density and total volatile compound content compared with wild-type peppermint; it also altered the volatile oil composition. In -overexpressing (OE) peppermint, the expression levels of several monoterpenoid synthase genes and glandular trichome development-related transcription factors-such as limonene synthase (), limonene-3-hydroxylase (), geranyl diphosphate synthase (), , and -exhibited varying degrees of alteration. overexpression resulted in both a change in expression of genes for terpenoid biosynthetic pathways which corresponded with an altered terpenoid profile in OE plants. In addition, peltate glandular trichome density was altered in the OE plants as well as the expression of genes for transcription factors that were shown to be involved in trichome development in plants.
唇萼薄荷是一种重要的香料作物和药用草本植物，具有很高的经济价值。该植物覆盖着盾状腺毛，负责挥发油的生物合成和分泌。植物非特异性脂质转运蛋白（nsLTPs）属于一个复杂的多基因家族，参与各种植物生理过程。在这里，我们从唇萼薄荷中克隆并鉴定了一个非特异性脂质转运蛋白基因（），它可能正向调节盾状腺毛密度和单萜代谢。该基因在唇萼薄荷的大多数组织中表达。在转基因唇萼薄荷中，由该基因启动子驱动的GUS信号在茎、叶和根中都能观察到；它也在腺毛中表达。该基因与质膜相关。与野生型薄荷相比，在薄荷（唇萼薄荷）中过表达该基因显著增加了盾状腺毛密度和总挥发性化合物含量；它还改变了挥发油的成分。在过表达该基因的（OE）薄荷中，几个单萜合酶基因和与腺毛发育相关的转录因子的表达水平，如柠檬烯合酶（）、柠檬烯-3-羟化酶（）、香叶基二磷酸合酶（）、以及，都表现出不同程度的变化。该基因的过表达导致了萜类生物合成途径相关基因表达的变化，这与OE植株中萜类谱的改变相对应。此外，OE植株中的盾状腺毛密度发生了改变，同时参与植物腺毛发育的转录因子基因的表达也发生了改变。
