Walker P D, McAllister J P
Exp Neurol. 1986 Aug;93(2):334-47. doi: 10.1016/0014-4886(86)90194-9.
We used the anterograde transport of HRP to analyze the nigrostriatal pathway after intrastriatal injections of kainic acid. A total volume of 1 microliter kainic acid (3 nM) was injected unilaterally into the neostriatum of adult rats. After 5, 10, or 35 days, HRP was injected into the ipsilateral substantia nigra. Sections stained for Nissl substance revealed that kainic acid damaged as much as three-quarters of the neostriatum. Lesion sites were characterized by gliosis and the absence of neurons. Alternate sections processed for HRP histochemistry and analyzed with bright- and dark-field microscopy revealed labeled axons and terminals in the lesion site. These findings were consistent in all three time periods. Much of the labeling was similar to that seen in neostriatal of control animals. However, the normal homogeneous pattern of the nigrostriatal terminal field was disrupted in all experimental groups, illustrated by changes in some labeling characteristics in the lesion site. These findings provide morphologic evidence for the preservation of much of the nigrostriatal pathway but indicate that some axons and their terminals may be altered after kainic acid injection.
我们运用辣根过氧化物酶（HRP）的顺行运输来分析纹状体内注射 kainic 酸后黑质纹状体通路的情况。将总量为 1 微升的 kainic 酸（3 纳摩尔）单侧注射到成年大鼠的新纹状体内。在 5 天、10 天或 35 天后，将 HRP 注射到同侧黑质。经尼氏物质染色的切片显示，kainic 酸损伤了多达四分之三的新纹状体。损伤部位的特征是胶质增生且无神经元。经 HRP 组织化学处理并用明视野和暗视野显微镜分析的交替切片显示，损伤部位有标记的轴突和终末。在所有三个时间段内，这些发现都是一致的。许多标记与对照动物新纹状体内所见的相似。然而，所有实验组中黑质纹状体终末场的正常均匀模式都被破坏了，损伤部位某些标记特征的变化说明了这一点。这些发现为黑质纹状体通路的大部分得以保留提供了形态学证据，但表明在注射 kainic 酸后，一些轴突及其终末可能会发生改变。
