应用即时检测方法调查北卡罗来纳州和宾夕法尼亚州黑熊体内弓形体抗体的流行情况。

USE OF A POINT OF CARE TEST TO DETERMINE THE PREVALENCE OF ANTIBODIES TO TOXOPLASMA GONDII IN BLACK BEARS FROM NORTH CAROLINA AND PENNSYLVANIA.

机构信息

Department of Forestry and Environmental Resources, Fisheries, Wildlife, and Conservation Biology, North Carolina State University, Raleigh, North Carolina 27606.

Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, Tennessee 37996.

出版信息

J Parasitol. 2023 May 1;109(3):221-224. doi: 10.1645/22-72.

DOI:10.1645/22-72

PMID:37327396

Abstract

Toxoplasma gondii is an important protozoan parasite of humans and animals throughout the world. Black bears are among the animals with the highest seroprevalence of T. gondii in the United States. A rapid point of care (POC) test is commercially available to detect antibodies to T. gondii in humans. We evaluated the utility of the POC test to detect anti-T. gondii antibodies in 100 wild black bears from North Carolina (n = 50) and Pennsylvania (n = 50). In a blind study, sera were tested by the POC test, and results were compared to the modified agglutination test (MAT). Overall, anti-T. gondii antibodies were detected in 76% (76/100) black bears by both MAT and POC tests. One false positive and one false negative result in the POC test were obtained in bears from Pennsylvania. The sensitivity and specificity of the POC test were both 99% when compared to the MAT. Results from our study indicate the POC test could be a useful screening tool for serological surveillance of T. gondii in black bears.

摘要

刚地弓形虫是一种重要的全球人类和动物的原生动物寄生虫。黑熊是美国弓形虫血清阳性率最高的动物之一。一种快速的即时检测（POC）测试已经商业化，可以检测人类对弓形虫的抗体。我们评估了该 POC 测试在北卡罗来纳州（n=50）和宾夕法尼亚州（n=50）的 100 只野生黑熊中检测抗弓形虫抗体的效用。在一项盲法研究中，通过 POC 测试检测血清，结果与改良凝集试验（MAT）进行比较。总体而言，MAT 和 POC 测试均检测到 76%（76/100）的黑熊存在抗弓形虫抗体。在来自宾夕法尼亚州的熊中，POC 测试获得了一个假阳性和一个假阴性结果。与 MAT 相比，POC 测试的灵敏度和特异性均为 99%。我们的研究结果表明，POC 测试可能是一种有用的血清学监测工具，用于监测黑熊中的弓形虫。