Costa E Silva Scarpa Pereira Carla, Perazzo Fábio Ferreira, Bianco de Souza Gustavo Henrique, Affonso Fonseca Fernando Luiz, Bonilha Dezena Renan Marcel, Gois Ruiz Ana Lúcia Tasca, Pires Rosa Paulo César
Faculty of Pharmaceutical Sciences, University of Campinas, Cidade Universitária Zeferino Vaz, 13081-970, Campinas, SP, Brazil.
Department of Exact Sciences and Earth, Federal University of São Paulo, Rua Arthur Riedel 275, Jardim Eldorado, 09072-270, Diadema, São Paulo, Brazil.
Ann Pharm Fr. 2023 Nov;81(6):950-967. doi: 10.1016/j.pharma.2023.06.002. Epub 2023 Jun 15.
The Pipercubeba, it is one spice, widely consumed in Europe, which has several bioactive molecules, between those a lignan named cubebin. Cubebin has several known biological activities, such as analgesic activity and anti-inflammatory, trypanocidal activity, leishmanicidal and antitumor activity. The objective of this study was to evaluate the antiproliferative activity "in vitro" cubebin in eight different human tumor cell lines. It was fully characterized by IR analysis, NMR, mass spectrometry, DSC, TGA, residual solvent and elemental analysis. The antitumor activity of cubebin was evaluated "in vitro" on eight different human tumor cell lineages. Cubebin showed GI≤30μg/mL for lineage cell U251 (glioma CNS), 786-0 (kidney), PC-3 (prostate), HT-29 (colon rectum). For K562 cells (leukemia), cubebin presented GI≤to 4.0mg/mL. For the other lineages cells, MCF-7 (breast) and NCI-H460 to cubebin can be considered inactive because of GI>250mg/mL. Analyzing the selectivity index for cubebin, it can be observed that high selectivity of cubebin to K562 lineage cells (leukemia). Analyzing the cytotoxic potential of cubebin was observed that probably acts cubebin altering metabolism, inhibiting cell growth - a cytostatic effect, showing no cytocidal effect on any lineage cell.
