Instituto de Salud y Ambiente del Litoral (ISAL, UNL-CONICET), Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina; Cátedra de Patología Humana, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
Laboratorio de Desarrollo Analítico y Quimiometría (LADAQ), Cátedra de Química Analítica I, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
Environ Toxicol Pharmacol. 2023 Aug;101:104184. doi: 10.1016/j.etap.2023.104184. Epub 2023 Jun 14.
This study aimed to assess whether perinatal exposure to propiconazole (PRO), glyphosate (GLY) or their mixture (PROGLY) alters key endocrine pathways and the development of the male rat mammary gland. To this end, pregnant rats were orally exposed to vehicle, PRO, GLY, or a mixture of PRO and GLY from gestation day 9 until weaning. Male offspring were euthanized on postnatal day (PND) 21 and PND60. On PND21, GLY-exposed rats showed reduced mammary epithelial cell proliferation, whereas PRO-exposed ones showed increased ductal p-Erk1/2 expression without histomorphological alterations. On PND60, GLY-exposed rats showed reduced mammary gland area and estrogen receptor alpha expression and increased aromatase expression, whereas PRO-exposed ones showed enhanced lobuloalveolar development and increased lobular hyperplasia. However, PROGLY did not modify any of the endpoints evaluated. In summary, PRO and GLY modified the expression of key molecules and the development of the male mammary gland individually but not together.
本研究旨在评估围产期暴露于丙环唑（PRO）、草甘膦（GLY）或其混合物（PROGLY）是否会改变关键的内分泌途径和雄性大鼠乳腺的发育。为此，妊娠大鼠从妊娠第 9 天到断奶期间经口暴露于载体、PRO、GLY 或 PRO 和 GLY 的混合物中。雄性后代于出生后第 21 天（PND21）和第 60 天（PND60）处死。在 PND21 时，暴露于 GLY 的大鼠显示乳腺上皮细胞增殖减少，而暴露于 PRO 的大鼠显示导管 p-Erk1/2 表达增加而没有组织形态学改变。在 PND60 时，暴露于 GLY 的大鼠显示乳腺面积和雌激素受体 alpha 表达减少，芳香化酶表达增加，而暴露于 PRO 的大鼠显示小叶泡状发育增强和小叶增生增加。然而，PROGLY 没有改变评估的任何终点。总之，PRO 和 GLY 单独但不共同改变了关键分子的表达和雄性乳腺的发育。
