Lab. de Toxicología, Depto. de Fisiopatología, Facultad de Ciencias Veterinarias, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET-UNCPBA, Tandil, Bs. As, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Bs. As, Argentina.
Vet Res Commun. 2023 Dec;47(4):1963-1972. doi: 10.1007/s11259-023-10150-w. Epub 2023 Jun 17.
Bovine alphaherpesvirus 1 (BoAHV-1) predisposes cattle to respiratory secondary bacterial infections, which can be treated with the broad-spectrum antibiotic fosfomycin. This drug also suppresses NF-kB activity and pro-inflammatory responses. Therefore, cattle may be exposed to an interaction between the virus and the antibiotic which may have effects on it. The aim of this study was to determine the effect of calcium fosfomycin (580 µg/mL) on BoAHV-1 (moi = 0.1) replication. Two cell lines (MDBK and SH-SY5Y) were used in this study. Our results show that fosfomycin has novel properties. By MTT assay we have shown that it is non-cytotoxic for any of the cell lines. Extracellular and intracellular viral titers demonstrated that fosfomycin has a cell-type and time-dependent effect on BoAHV-1 replication. By direct immunofluorescence it was shown that it reduces the timing of BoAHV-1 protein expression, and by qPCR, we found that its effect on NF-kB mRNA expression depends on the cell type.
牛α疱疹病毒 1（BoAHV-1）使牛易患呼吸道继发细菌性感染，可使用广谱抗生素磷霉素治疗。这种药物还能抑制 NF-κB 活性和促炎反应。因此，牛可能会受到病毒和抗生素相互作用的影响，而这种相互作用可能对其产生影响。本研究旨在确定钙磷霉素（580μg/mL）对 BoAHV-1（感染复数 moi=0.1）复制的影响。本研究使用了两种细胞系（MDBK 和 SH-SY5Y）。我们的结果表明，磷霉素具有新的特性。通过 MTT 测定，我们证明它对任何一种细胞系均无细胞毒性。细胞外和细胞内病毒滴度表明，磷霉素对 BoAHV-1 复制具有细胞类型和时间依赖性的影响。通过直接免疫荧光，我们发现它可以减少 BoAHV-1 蛋白表达的时间，通过 qPCR，我们发现其对 NF-κB mRNA 表达的影响取决于细胞类型。
