College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi, 030801, China.
College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi, 030801, China; Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin, 300071, China.
Talanta. 2023 Dec 1;265:124802. doi: 10.1016/j.talanta.2023.124802. Epub 2023 Jun 14.
In this paper, an analysis method for chlorpyrifos (CPF) in cereal samples was proposed using dispersive liquid‒liquid microextraction combined with an enzyme-linked immunosorbent assay. In the dispersive liquid‒liquid microextraction, deep eutectic solvents and fatty acids were used as solvents to extract, purify, and concentrate CPF in cereals. In the enzyme-linked immunosorbent assay, gold nanoparticles were utilized to enrich and conjugate more antibodies and horseradish peroxidase, while magnetic beads were used as solid supports to amplify the signal and shorten the detection time of CPF. The linearity range was 0.002-1 μg kg, and the limit of detection was 0.0006 μg kg. The extraction recoveries were 86.7-99.9% with a relative standard deviation of less than 7.0%. The proposed method was successfully used to analyze CPF in cereal samples (rice, wheat, maize, and millet) and has prospects for the pretreatment and detection of CPF residues in other food samples.
本文提出了一种利用分散液-液微萃取结合酶联免疫吸附法分析谷物样品中毒死蜱（CPF）的方法。在分散液-液微萃取中，深共晶溶剂和脂肪酸被用作溶剂，以萃取、净化和浓缩谷物中的 CPF。在酶联免疫吸附法中，金纳米粒子被用于富集和结合更多的抗体和辣根过氧化物酶，而磁性珠则被用作固体载体来放大信号并缩短 CPF 的检测时间。该方法的线性范围为 0.002-1μgkg，检出限为 0.0006μgkg。萃取回收率为 86.7-99.9%，相对标准偏差小于 7.0%。该方法成功地用于分析谷物样品（大米、小麦、玉米和小米）中的 CPF，并有望用于其他食品样品中 CPF 残留的预处理和检测。
