Department of Bioprocesses and Materials Engineering, School of Chemical Engineering, University of Campinas (UNICAMP), São Paulo, Brazil.
Department of Bioprocesses and Materials Engineering, School of Chemical Engineering, University of Campinas (UNICAMP), São Paulo, Brazil.
Int J Biol Macromol. 2023 Jul 31;244:125388. doi: 10.1016/j.ijbiomac.2023.125388. Epub 2023 Jun 15.
Pathogen agents, such as bacteria and virus, can contaminate plastic surfaces, particularly those used in food packaging. This study proposed to prepare a polyelectrolyte film with antiviral and antibacterial activity based on sodium alginate (SA) and poly(diallyldimethylammonium chloride) (PDADMAC), a cationic polymer with sanitizing properties. In addition, the physicochemical properties of the polyelectrolyte films were also evaluated. The polyelectrolyte films showed continuous, compact, and crack-free structures. The FTIR analysis confirmed the ionic interaction between SA and PDADMAC. Adding PDADMAC significantly affected the mechanical properties of the films (p < 0.05), increasing the maximum tensile strength (from 8.66 ± 1.55 MPa to 18.1 ± 1.77 MPa). However, polyelectrolyte films showed higher water vapor permeability values due to the strong hydrophilicity of PDADMAC, representing a 43 % average increase compared with the control film. Also, thermal stability improved with the incorporation of PDADMAC. The selected polyelectrolyte film inactivated 99.8 % of SARS-CoV-2 after 1 min in direct contact with the virus, in addition to having an inhibitory effect against Staphylococcus aureus and Escherichia coli bacteria. Therefore, this study demonstrated the efficacy of using PDADMAC in the preparation of polyelectrolyte sodium alginate-based films with improvements in physicochemical properties and especially with antiviral activity against SARS-CoV-2.
病原体（如细菌和病毒）可污染塑料表面，特别是那些用于食品包装的塑料表面。本研究拟基于海藻酸钠（SA）和聚二烯丙基二甲基氯化铵（PDADMAC）（一种具有消毒性能的阳离子聚合物）制备具有抗病毒和抗菌活性的聚电解质膜。此外，还评估了聚电解质膜的理化性质。聚电解质膜具有连续、致密、无裂纹的结构。FTIR 分析证实了 SA 和 PDADMAC 之间的离子相互作用。添加 PDADMAC 显著影响了膜的机械性能（p<0.05），最大拉伸强度从 8.66±1.55 MPa 增加到 18.1±1.77 MPa。然而，由于 PDADMAC 的强亲水性，聚电解质膜表现出更高的水蒸气透过率值，与对照膜相比，平均增加了 43%。此外，随着 PDADMAC 的掺入，热稳定性得到提高。所选聚电解质膜在与病毒直接接触 1 分钟后，可使 99.8%的 SARS-CoV-2 失活，此外对金黄色葡萄球菌和大肠杆菌也有抑制作用。因此，本研究证明了在制备海藻酸钠基聚电解质膜时使用 PDADMAC 的有效性，改善了物理化学性质，特别是具有抗 SARS-CoV-2 的抗病毒活性。
