Yildiz Technical University, Chemical and Metallurgical Engineering Faculty, Food Engineering Department, Davutpasa Campus, 34210 Istanbul, Turkey.
Yildiz Technical University, Chemical and Metallurgical Engineering Faculty, Food Engineering Department, Davutpasa Campus, 34210 Istanbul, Turkey.
Int J Biol Macromol. 2023 Jul 31;244:125240. doi: 10.1016/j.ijbiomac.2023.125240. Epub 2023 Jun 8.
In this study, sodium alginate based biodegradable films were prepared by the supplementation with postbiotics of Lactiplantibacillus plantarum subsp. plantarum (L. plantarum) W2 strain and the effect of probiotics (probiotic-SA film) and postbiotics (postbiotic-SA film) incorporation on physical, mechanical (tensile strength and elongation at break), barrier (oxygen and water vapor permeability), thermal and antimicrobial properties of the films was investigated. The pH, titratable acidity and brix of the postbiotic was 4.02, 1.24 % and 8.37, respectively while gallic acid, protocatechuic acid, myricetin and catechin were the major phenolic compounds. Mechanical and barrier properties of the alginate-based films were improved by probiotic or postbiotic supplementation while postbiotic showed a more pronounced (P < 0.05) effect. Thermal analysis showed that postbiotics supplementation increased thermal stability of the films. In FTIR spectra, the absorption peaks at 2341 and 2317 cm for probiotic-SA and postbiotic-SA edible films confirmed the incorporation of probiotics/postbiotics of L. plantarum W2 strain. Postbiotic supplemented films showed strong antibacterial activity against gram-positive (L. monocytogenes, S. aureus and B. cereus) and gram-negative bacterial (E. coli O157:H7) strains while probiotic-SA films did not show any antibacterial effect against the test pathogens. SEM images revealed that the supplementation of postbiotics provided a rougher and rigid film surface. Overall, this paper brought a new perspective for development of novel active biodegradable films by incorporation of postbiotics with improved performance.
在这项研究中，通过添加植物乳杆菌（Lactiplantibacillus plantarum）W2 菌株的后生元，制备了基于海藻酸钠的可生物降解薄膜，并研究了益生菌（益生菌-SA 薄膜）和后生元（后生元-SA 薄膜）对薄膜的物理、机械（拉伸强度和断裂伸长率）、阻隔（氧气和水蒸气透过性）、热和抗菌性能的影响。后生元的 pH 值、可滴定酸度和糖度分别为 4.02、1.24%和 8.37，而没食子酸、原儿茶酸、杨梅素和儿茶素是主要的酚类化合物。通过益生菌或后生元的添加，海藻酸钠基薄膜的机械和阻隔性能得到了改善，而后生元的效果更为显著（P<0.05）。热分析表明，后生元的添加提高了薄膜的热稳定性。在 FTIR 光谱中，益生菌-SA 和后生元-SA 可食用薄膜在 2341 和 2317cm 处的吸收峰证实了 L. plantarum W2 菌株的益生菌/后生元的掺入。后生元补充薄膜对革兰氏阳性（单核细胞增生李斯特菌、金黄色葡萄球菌和蜡状芽孢杆菌）和革兰氏阴性细菌（E. coli O157:H7）菌株表现出强烈的抗菌活性，而益生菌-SA 薄膜对测试的病原体没有任何抗菌作用。SEM 图像显示，后生元的添加提供了更粗糙和刚性的薄膜表面。总的来说，本文通过添加后生元提供了一种新的视角，为开发具有改善性能的新型活性可生物降解薄膜提供了思路。
