Department of Chemistry, Visva-Bharati University, Santiniketan 731235, India.
S. N. Pradhan Centre for Neurosciences, University of Calcutta, Kolkata 700019, India.
J Photochem Photobiol B. 2023 Aug;245:112747. doi: 10.1016/j.jphotobiol.2023.112747. Epub 2023 Jun 14.
Cysteine is directly associated with a wide range of biological processes. Besides its essential role in protein synthesis, cysteine undergoes a variety of post-translational modifications which modulate several physiological processes. Dysregulated cysteine metabolism is associated with several neurodegenerative disorders. Accordingly, restoring cysteine balance has therapeutic benefits. It is therefore essential to detect the presence of endogenous free cysteine in order to understand different physiological modes of action inside the cell. Here, a carbazole-pyridoxal conjugate system (CPLC) has been developed to detect endogenous free cysteine in the liver and kidney of an adult zebrafish. In consequence, we have also determined the fluorescence intensity statistics of zebrafish kidney and liver images. CPLC interacts in a very fascinating way with two cysteine molecules through chemodosimetric and chemosensing approaches which are conclusively proved by different spectroscopic analyses (UV-vis, fluorescence, NMR) and theoretical calculations (DFT). The detection limit of CPLC towards cysteine is 0.20 μM. Moreover, this preliminary experiment has been done using HuH-7 cell line to check the permeability of CPLC, interaction with cysteine intracellularly, and assessment of the toxicity of CPLC, if any, before performing details in-vivo experiments in zebrafish model.
半胱氨酸直接参与广泛的生物过程。除了在蛋白质合成中的重要作用外，半胱氨酸还经历了多种翻译后修饰，调节了多种生理过程。半胱氨酸代谢失调与多种神经退行性疾病有关。因此，恢复半胱氨酸平衡具有治疗益处。因此，检测细胞内源性游离半胱氨酸的存在对于了解不同的生理作用模式至关重要。在这里，开发了一种咔唑-吡哆醛缀合物系统 (CPLC) 来检测成年斑马鱼肝脏和肾脏中的内源性游离半胱氨酸。因此，我们还确定了斑马鱼肾脏和肝脏图像的荧光强度统计数据。CPLC 通过化学计量和化学传感方法与两个半胱氨酸分子以非常迷人的方式相互作用，这通过不同的光谱分析 (UV-vis、荧光、NMR) 和理论计算 (DFT) 得到了明确证明。CPLC 对半胱氨酸的检测限为 0.20 μM。此外，这项初步实验使用 HuH-7 细胞系进行，以检查 CPLC 的通透性、与细胞内半胱氨酸的相互作用以及 CPLC 的毒性评估（如果有），然后在斑马鱼模型中进行详细的体内实验。
