Chen Xin, Wang Qing-Wan, Zhan Tong-Ling, Sun Yue, Zhang Xiao-Rui, Liu Kun, Liu Xiao-Wu
School of Materials and Chemical Engineering, West Anhui University, Lu'an, Anhui, 237012, China.
Anhui Province Key Laboratory of Conservation and Utilization for Dabie Mountain Special Bio-Resources, West Anhui University, Lu'an, 237012, China.
J Fluoresc. 2025 Jan 11. doi: 10.1007/s10895-024-04124-7.
Nitrogen@Carbon quantum dots (N@CQDs) are prepared using microwave hydrothermal method, and polyvinylpyrrolidone (PVP) and melamine are used as mixed C source and N source. Microwave reaction conditions of preparing the N@CQDs are 170 ℃ and 3 h. This N@CQDs are are used as fluorescence probe for detection of amino acids. The N@CQDs can recognize L-alanine in water solution, and simultaneously recognize both L-methionine and L-cysteine in dimethyl sulfoxide (DMSO) solution. The linear ranges for L-alanine, L-methionine and L-cysteine are 0-15 µM, 2-50 µM and 0-17 µM, respectively. While limits of detection (LOD) for them are 11.092 nM, 7.8067 nM and 0.67476 nM, respectively. Compared to presently available detection methods, as-prepared N@CQDs have the advantage of high sensitivity and selectivity. Furthermore, coexistence of other amino acids has minimal interference in the detection of L-alanine, L-methionine and L-cysteine. For the as-prepared N@CQDs, solvent plays important roles in influencing the identification of amino acid types and detection sensitivity.
采用微波水热法制备氮掺杂碳量子点（N@CQDs），以聚乙烯吡咯烷酮（PVP）和三聚氰胺作为混合碳源和氮源。制备N@CQDs的微波反应条件为170℃、3小时。将此N@CQDs用作检测氨基酸的荧光探针。该N@CQDs能识别水溶液中的L-丙氨酸，同时能在二甲基亚砜（DMSO）溶液中识别L-蛋氨酸和L-半胱氨酸。L-丙氨酸、L-蛋氨酸和L-半胱氨酸的线性范围分别为0 - 15μM、2 - 50μM和0 - 17μM。而它们的检测限（LOD）分别为11.092 nM、7.8067 nM和0.67476 nM。与现有检测方法相比，所制备的N@CQDs具有高灵敏度和高选择性的优点。此外，其他氨基酸的共存对L-丙氨酸、L-蛋氨酸和L-半胱氨酸的检测干扰极小。对于所制备的N@CQDs，溶剂在影响氨基酸类型识别和检测灵敏度方面起着重要作用。
