School of Life Science and Engineering,Southwest Jiaotong University, Chengdu 610031, China; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
J Pharm Biomed Anal. 2023 Sep 20;234:115528. doi: 10.1016/j.jpba.2023.115528. Epub 2023 Jun 14.
Pulsatilla chinensis (P.chinensis) is a traditional Chinese medicine used for the treatment of intestinal amebiasis diseases, vaginal trichomoniasis and bacterial infections. Tritepenoid saponins were important components of P.chinensis. Therefore, we asssessmented expression profiling of triterpenoids in different fresh tissues of P.chinensis by ultra high performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) and ultra high performance liquid chromatography coupled to triple quadrupole mass spectrometry (UHPLC-QQQ-MS). Firstly, we identified 132 triterpenoids, including 119 triterpenoid saponins, 13 triterpenoid acids and forty seven of them were first determined in Pulsatilla genus, including new aglycones and new ways of rhamnose linking to the aglycone. Secondly, we established the analytical method to analysis triterpenoids content of P.chinensis and comprehensively verified the analytical method by linearity, precision, repeatability, stability and recovery. At last, we quantified 119 triterpenoids simultaneously based on UHPLC-QQQ-MS. The results show that the types and contents of triterpenoids had obvious tissue distribution. New components like rhamnose directly linked to the aglycone mainely distributed in aboveground tissues. Additionally, We identified 15 chemical ingredients as differential components between the aboveground and underground tissues of P.chinensis. This study provides an efficient analysis strategy for the qualitative and quantitative analysis of triterpenoids in P.chinensis even in other traditional Chinese medicines. At the same time, it provides important informations to explain the biosynthetic pathway of triterpenoid saponins in P.chinensis.
白头翁（Pulsatilla chinensis）是一种传统中药，用于治疗肠道阿米巴病、阴道滴虫病和细菌感染。三萜皂苷是白头翁的重要组成部分。因此，我们通过超高效液相色谱-四极杆飞行时间质谱（UHPLC-Q-TOF-MS）和超高效液相色谱-三重四极杆质谱（UHPLC-QQQ-MS）评估了不同新鲜组织中白头翁三萜的表达谱。首先，我们鉴定了 132 种三萜类化合物，包括 119 种三萜皂苷、13 种三萜酸，其中 47 种为白头翁属首次确定的化合物，包括新的苷元和新的鼠李糖与苷元的连接方式。其次，我们建立了分析白头翁三萜含量的分析方法，并通过线性、精密度、重复性、稳定性和回收率对分析方法进行了全面验证。最后，我们基于 UHPLC-QQQ-MS 同时定量了 119 种三萜类化合物。结果表明，三萜类化合物的类型和含量具有明显的组织分布。新的鼠李糖直接与苷元相连的成分主要分布在上部组织中。此外，我们还鉴定了 15 种化学成分作为白头翁地上和地下组织之间的差异成分。本研究为白头翁甚至其他中药中三萜类化合物的定性和定量分析提供了有效的分析策略。同时，为解释白头翁三萜皂苷的生物合成途径提供了重要信息。
