超高效液相色谱-四极杆飞行时间质谱联用（UPLC/Q-TOF-MS/MS）法对大鼠体内白头翁皂苷D的代谢物谱分析
Metabolites profiling of Pulsatilla saponin D in rat by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS/MS).
作者信息
Ouyang Hui, Zhou Maofu, Guo Yicheng, He Mingzhen, Huang Hesong, Ye Xide, Feng Yulin, Zhou Xin, Yang Shilin
机构信息
Jiangxi University of Traditional Chinese Medicine, No. 56 Yangmin Road, Nanchang 330006, PR China; Guangzhou University of Chinese Medicine, No. 232 Waihuandong Road, Guangzhou 510405, PR China.
Jiangxi University of Traditional Chinese Medicine, No. 56 Yangmin Road, Nanchang 330006, PR China.
出版信息
Fitoterapia. 2014 Jul;96:152-8. doi: 10.1016/j.fitote.2014.05.002. Epub 2014 May 14.
Pulsatilla saponin D, an antitumor substance isolated from traditional Chinese herbal medicine Pulsatilla chinensis (Bge.) Regel, is a promising candidate for new drug development. The purpose of the present study is to establish a simple and practical strategy for the metabolite profiling of Pulsatilla saponin D in vivo. A total of 18 metabolites were identified in rat plasma, urine and feces samples based on MS and MS/MS data by using ESI-Q-TOF-MS/MS, and eight of them (M11-M18) were reported for the first time. The results indicated that deglycosylation, dehydrogenation, hydroxylation and sulfation were the major metabolic transformations of Pulsatilla saponin D in vivo. This study has improved our understanding of the metabolic fate of Pulsatilla saponin D in vivo, and the information gained from the current study is relevant to the pharmacological activity of Pulsatilla saponin D.
白头翁皂苷D是从传统中药白头翁中分离出的一种抗肿瘤物质，是新药开发的一个有前景的候选药物。本研究的目的是建立一种简单实用的体内白头翁皂苷D代谢物谱分析策略。利用电喷雾四级杆-飞行时间串联质谱（ESI-Q-TOF-MS/MS），基于质谱和串联质谱数据，在大鼠血浆、尿液和粪便样本中总共鉴定出18种代谢物，其中8种（M11-M18）为首次报道。结果表明，去糖基化、脱氢、羟基化和硫酸化是白头翁皂苷D在体内的主要代谢转化方式。本研究增进了我们对白头翁皂苷D在体内代谢命运的理解，并且从当前研究中获得的信息与白头翁皂苷D的药理活性相关。
Zotero 插件