Li Shu, Qi Bao, Peng Xueyan, Wang Wei, Wang Wan, Liu Pu, Liu Bao, Peng Zhanwu, Wang Qi, Li Yu
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China; Key Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal University, Changchun 130024, China.
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China.
Eur J Protistol. 2023 Aug;90:125991. doi: 10.1016/j.ejop.2023.125991. Epub 2023 Jun 1.
More than 1272 myxomycetes species have been described, accounting for more than half of all Amoebozoa species. However, the genome size of only three myxomycetes species has been reported. Therefore, we used flow cytometry to present an extensive survey and a phylogeny-based analysis of genome size and GC content evolution in 144 myxomycetes species. The genome size of myxomycetes ranged from 18.7 Mb to 470.3 Mb, and the GC content ranged from 38.7% to 70.1%. Bright-spored clade showed larger genome sizes and more intra-order genome size variations than the dark-spored clade. GC content and genome size were positively correlated in both bright-spored and dark-spored clades, and spore size was positively correlated with genome size and GC content in the bright-spored clade. We provided the first genome size data set in Myxomycetes, and our results will provide helpful information for future Myxomycetes studies, such as genome sequencing.
已描述的黏菌物种超过1272种，占所有变形虫物种的一半以上。然而，仅报道了三种黏菌物种的基因组大小。因此，我们使用流式细胞术对144种黏菌物种的基因组大小和GC含量进化进行了广泛的调查和基于系统发育的分析。黏菌的基因组大小在18.7 Mb至470.3 Mb之间，GC含量在38.7%至70.1%之间。与暗孢子类群相比，亮孢子类群显示出更大的基因组大小和更多的目内基因组大小变异。在亮孢子和暗孢子类群中，GC含量与基因组大小均呈正相关，在亮孢子类群中，孢子大小与基因组大小和GC含量呈正相关。我们提供了黏菌中的首个基因组大小数据集，我们的结果将为未来黏菌研究（如基因组测序）提供有用信息。
