Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, 510070, PR China.
Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, 510070, PR China; School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, Guangdong, PR China.
J Environ Manage. 2023 Oct 15;344:118396. doi: 10.1016/j.jenvman.2023.118396. Epub 2023 Jun 16.
Antibiotic resistance in drinking water has received increasing attention in recent years. In this study, the occurrence and abundance of antibiotic resistance genes (ARGs) in a drinking water treatment plant (DWTP) was comprehensively investigated using metagenomics. Bioinformatics analysis showed that 381 ARG subtypes belonging to 15 ARG types were detected, and bacitracin had the highest abundance (from 0.26 × 10 to 0.86 copies/cell), followed by multidrug (from 0.57 × 10 to 0.47 copies/cell) and sulfonamide (from 0.83 × 10 to 0.35 copies/cell). Additionally, 933 ARG-carrying contigs (ACCs) were obtained from the metagenomic data, among which 153 contigs were annotated as pathogens. The most abundant putative ARG host was Staphylococcus (7.9%), which most frequently carried multidrug ARGs (43.2%). Additionally, 38 high-quality metagenome-assembled genomes (MAGs) were recovered, one of which was identified as Staphylococcus aureus (Bin.624) and harboured the largest number of ARGs (n = 16). Using the cultivation technique, 60 isolates were obtained from DWTP samples, and Staphylococcus spp. (n = 11) were found to be dominant in all isolates, followed by Bacillus spp. (n = 17). Antimicrobial susceptibility testing showed that most Staphylococcus spp. were multidrug resistant (MDR). These results deepen our understanding of the distribution profiles of ARGs and antibiotic resistant bacteria (ARB) in DWTPs for potential health risk evaluation. Our study also highlights the need for new and efficient water purification technologies that can be introduced and applied in DWTPs.
近年来，饮用水中的抗生素耐药性受到了越来越多的关注。在这项研究中，使用宏基因组学全面调查了饮用水处理厂（DWTP）中抗生素耐药基因（ARGs）的发生和丰度。生物信息学分析表明，检测到属于 15 种 ARG 类型的 381 种 ARG 亚型，其中杆菌肽的丰度最高（从 0.26×10 到 0.86 拷贝/细胞），其次是多药（从 0.57×10 到 0.47 拷贝/细胞）和磺胺类（从 0.83×10 到 0.35 拷贝/细胞）。此外，从宏基因组数据中获得了 933 个 ARG 携带的连续体（ACCs），其中 153 个连续体被注释为病原体。最丰富的假定 ARG 宿主是葡萄球菌（7.9%），其最常携带多药 ARGs（43.2%）。此外，还回收了 38 个高质量的宏基因组组装基因组（MAGs），其中一个被鉴定为金黄色葡萄球菌（Bin.624），携带最多的 ARGs（n=16）。使用培养技术从 DWTP 样本中获得了 60 个分离株，所有分离株中均以葡萄球菌属（n=11）为主，其次是芽孢杆菌属（n=17）。药敏试验表明，大多数葡萄球菌属均为多药耐药（MDR）。这些结果加深了我们对 DWTP 中 ARGs 和抗生素耐药细菌（ARB）分布特征的理解，有助于进行潜在的健康风险评估。我们的研究还强调了需要引入和应用于 DWTP 的新型高效水净化技术。
