Department of Medical and Surgical Sciences for Children & Adults, University, Hospital of Modena and Reggio Emilia, Modena, Italy.
Unit of Pathology, Azienda Ospedaliero-Universitaria Policlinico, Modena, Italy.
Gynecol Endocrinol. 2023 Jun 9;39(1):2224457. doi: 10.1080/09513590.2023.2224457.
To investigate the expression and localization of Vasorin (Vasn) in human female reproductive system. The presence of Vasorin was evaluated by RT-PCR and immunoblotting analyses in patient-derived endometrial, myometrial and granulosa cells (GCs) primary cultures. Immunostaining analyses were performed to detect Vasn localization in primary cultures and in ovarian and uterine tissues. Vasn mRNA was detected in patient-derived endometrial, myometrial and GCs primary cultures without significant differences at the transcript level. Otherwise, immunoblotting analysis showed that Vasn protein levels were significantly higher in GCs than proliferative endometrial stromal cells (ESCs) and myometrial cells. Immunohistochemistry performed in ovarian tissues revealed that Vasn was expressed in the GCs of ovarian follicles at different stages of development with a higher immunostaining signal in mature ovarian follicles such as the antral follicle or on the surface of cumulus oophorus cells than in early-stage follicles. The immunostaining of uterine tissues showed that Vasn was expressed in the proliferative stroma endometrium while it was significantly less expressed in the secretory endometrium. Conversely, no protein immunoreactivity was revealed in health myometrial tissue. Our results revealed the presence of Vasn in the ovary and the endometrium. The pattern of Vasn expression and distribution suggests that this protein may have a role in the regulation of processes such as folliculogenesis, oocyte maturation, and endometrial proliferation.
为了研究 Vasorin（Vasn）在人体女性生殖系统中的表达和定位。通过 RT-PCR 和免疫印迹分析评估了 Vasorin 在患者来源的子宫内膜、子宫肌层和颗粒细胞（GCs）原代培养物中的存在。免疫染色分析用于检测原代培养物以及卵巢和子宫组织中 Vasn 的定位。Vasn mRNA 在患者来源的子宫内膜、子宫肌层和 GCs 原代培养物中均有检测到，转录水平无显著差异。然而，免疫印迹分析显示，GCs 中的 Vasn 蛋白水平明显高于增殖期子宫内膜基质细胞（ESCs）和子宫肌层细胞。在卵巢组织中进行的免疫组织化学显示，Vasn 在不同发育阶段的卵巢卵泡的 GCs 中表达，在成熟卵泡（如窦卵泡或卵丘细胞表面）中的免疫染色信号高于早期卵泡。子宫组织的免疫染色显示，Vasn 在增殖期子宫内膜基质中表达，而在分泌期子宫内膜中表达明显减少。相反，健康的子宫肌层组织中没有蛋白免疫反应性。我们的结果表明 Vasn 存在于卵巢和子宫内膜中。Vasn 的表达和分布模式表明，该蛋白可能在卵泡发生、卵母细胞成熟和子宫内膜增殖等过程的调节中发挥作用。
