对感染了小鼠锥虫的小鼠血液进行治疗活性的体外测定。
In vitro assay for curative activity in blood of mice infected with Trypanosoma musculi.
作者信息
Wechsler D S, Kongshavn P A
出版信息
Infect Immun. 1986 Aug;53(2):240-4. doi: 10.1128/iai.53.2.240-244.1986.
Abstract
An in vitro assay for curative antibody present in plasma of mice cured of Trypanosoma musculi is described. The assay involves the addition of plasma to a sample of infected blood, followed by hourly monitoring of the parasite count therein. Immune plasma effected a significant reduction in parasite number, whereas normal mouse plasma did not. Heat treatment of immune plasma for 30 min at 56 degrees C abolished its ability to effect any reduction. The assay is simple, rapid, and economical and correlates well with results of all in vivo studies performed to date.
摘要
本文描述了一种用于检测治愈了鼠锥虫的小鼠血浆中存在的治疗性抗体的体外试验。该试验包括将血浆加入到感染血液样本中，随后每小时监测其中的寄生虫数量。免疫血浆使寄生虫数量显著减少，而正常小鼠血浆则没有这种作用。将免疫血浆在56℃下热处理30分钟会消除其使寄生虫数量减少的能力。该试验简单、快速且经济，并且与迄今为止进行的所有体内研究结果相关性良好。
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