对感染了小鼠锥虫的小鼠血液进行治疗活性的体外测定。
In vitro assay for curative activity in blood of mice infected with Trypanosoma musculi.
作者信息
Wechsler D S, Kongshavn P A
出版信息
Infect Immun. 1986 Aug;53(2):240-4. doi: 10.1128/iai.53.2.240-244.1986.
Abstract
An in vitro assay for curative antibody present in plasma of mice cured of Trypanosoma musculi is described. The assay involves the addition of plasma to a sample of infected blood, followed by hourly monitoring of the parasite count therein. Immune plasma effected a significant reduction in parasite number, whereas normal mouse plasma did not. Heat treatment of immune plasma for 30 min at 56 degrees C abolished its ability to effect any reduction. The assay is simple, rapid, and economical and correlates well with results of all in vivo studies performed to date.
摘要
本文描述了一种用于检测治愈了鼠锥虫的小鼠血浆中存在的治疗性抗体的体外试验。该试验包括将血浆加入到感染血液样本中，随后每小时监测其中的寄生虫数量。免疫血浆使寄生虫数量显著减少，而正常小鼠血浆则没有这种作用。将免疫血浆在56℃下热处理30分钟会消除其使寄生虫数量减少的能力。该试验简单、快速且经济，并且与迄今为止进行的所有体内研究结果相关性良好。
相似文献
1
In vitro assay for curative activity in blood of mice infected with Trypanosoma musculi.对感染了小鼠锥虫的小鼠血液进行治疗活性的体外测定。
Infect Immun. 1986 Aug;53(2):240-4. doi: 10.1128/iai.53.2.240-244.1986.
2
Characterization of antibodies mediating protection and cure of Trypanosoma musculi infection in mice.介导小鼠肌肉锥虫感染保护和治愈作用的抗体的特性分析。
Infect Immun. 1985 Jun;48(3):787-94. doi: 10.1128/iai.48.3.787-794.1985.
3
Heat-labile IgG2a antibodies affect cure of Trypanosoma musculi infection in C57BL/6 mice.
J Immunol. 1986 Nov 1;137(9):2968-72.
4
Cure of Trypanosoma musculi infection by heat-labile activity in immune plasma.免疫血浆中热不稳定活性对鼠锥虫感染的治愈作用。
Infect Immun. 1984 Jun;44(3):756-9. doi: 10.1128/iai.44.3.756-759.1984.
5
Further characterization of the curative antibodies in Trypanosoma musculi infection.鼠锥虫感染中治疗性抗体的进一步特性研究。
Infect Immun. 1988 Sep;56(9):2379-84. doi: 10.1128/iai.56.9.2379-2384.1988.
6
Trypanosoma musculi: immunologic features of the anemia in infected mice.
Exp Parasitol. 1977 Oct;43(1):203-10. doi: 10.1016/0014-4894(77)90024-8.
7
Effects of antiserum to Trypanosoma cruzi on the uptake and rate of killing of vector-borne, metacyclic forms of the parasite by macrophages.
Int J Parasitol. 1985 Aug;15(4):409-13. doi: 10.1016/0020-7519(85)90026-8.
8
Failure to demonstrate a major role for Kupffer cells and radiosensitive leukocytes in immunoglobulin-mediated elimination of Trypanosoma musculi.未能证明库普弗细胞和放射敏感白细胞在免疫球蛋白介导的消除鼠锥虫过程中起主要作用。
Infect Immun. 1990 Jun;58(6):1971-8. doi: 10.1128/iai.58.6.1971-1978.1990.
9
Infection in Mice Critically Relies on Mannose Receptor-Mediated Arginase Induction by a KHC1 Kinesin H Chain Homolog.小鼠感染严重依赖于一种KHC1驱动蛋白重链同源物介导的甘露糖受体诱导的精氨酸酶。
J Immunol. 2017 Sep 1;199(5):1762-1771. doi: 10.4049/jimmunol.1700179. Epub 2017 Jul 24.
10
Autoradiography of blood forms of Trypanosoma musculi.
Can J Microbiol. 1972 Apr;18(4):533-4. doi: 10.1139/m72-081.
引用本文的文献
1
Further characterization of the curative antibodies in Trypanosoma musculi infection.鼠锥虫感染中治疗性抗体的进一步特性研究。
Infect Immun. 1988 Sep;56(9):2379-84. doi: 10.1128/iai.56.9.2379-2384.1988.
本文引用的文献
1
The four subclasses of IgG can be isolated from mouse serum by using Protein A-Sepharose.IgG的四个亚类可以通过使用蛋白A-琼脂糖凝胶从小鼠血清中分离出来。
Scand J Immunol. 1981 Oct;14(4):335-42. doi: 10.1111/j.1365-3083.1981.tb00573.x.
2
Cure of Trypanosoma musculi infection by heat-labile activity in immune plasma.免疫血浆中热不稳定活性对鼠锥虫感染的治愈作用。
Infect Immun. 1984 Jun;44(3):756-9. doi: 10.1128/iai.44.3.756-759.1984.
3
Trypanosoma musculi infection in B-cell-deficient mice.B细胞缺陷小鼠中的鼠锥虫感染。
Infect Immun. 1984 Apr;44(1):162-7. doi: 10.1128/iai.44.1.162-167.1984.
4
Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose.使用二乙氨基乙基纤维素从人和其他哺乳动物中分离涎源性锥虫。
Exp Parasitol. 1970 Dec;28(3):521-34. doi: 10.1016/0014-4894(70)90120-7.
5
6
7
Characterization of antibodies mediating protection and cure of Trypanosoma musculi infection in mice.介导小鼠肌肉锥虫感染保护和治愈作用的抗体的特性分析。
Infect Immun. 1985 Jun;48(3):787-94. doi: 10.1128/iai.48.3.787-794.1985.
8
The immunological response of CBA mice to Trypanosoma musculi: elimination of the parasite from the blood.
Int J Parasitol. 1975 Apr;5(2):231-4. doi: 10.1016/0020-7519(75)90034-x.
9
Chronic infection with Trypanosoma musculi in congenitally athymic nude mice.先天性无胸腺裸鼠的小鼠锥虫慢性感染。
Infect Immun. 1977 May;16(2):715-6. doi: 10.1128/iai.16.2.715-716.1977.
10
Thymus dependency of Trypanosoma musculi elimination from mice.
J Reticuloendothel Soc. 1977 Dec;22(6):605-8.
Zotero 插件