E受体的E玫瑰花结阻断效应研究。

Study on the E-rosette-blocking effects of E receptors.

作者信息

Gu S Q, Wasserman J

出版信息

Int Arch Allergy Appl Immunol. 1986;80(4):391-4. doi: 10.1159/000234086.

DOI:10.1159/000234086

Abstract

Lymphocytes were purified from 10 samples of normal peripheral blood. E receptors were detached by heating to +45 degrees C. The sheep red blood cells (SRBC) and 2-aminoethylisothiouronium bromide (AET)-treated SRBC (AET-SRBC) were incubated at +4 degrees C for 2 h, with E receptors. E receptors detached by heating blocked E-rosette formation with SRBC, but not with AET-SRBC. The results suggest that E receptors may exhibit different conformational structures or different molecules which are responsible for E-rosette formation and E-AET-rosette formation, respectively.

摘要

从10份正常外周血样本中纯化淋巴细胞。通过加热至45摄氏度去除E受体。将绵羊红细胞（SRBC）和经2-氨基乙基异硫脲溴化物（AET）处理的SRBC（AET-SRBC）与E受体在4摄氏度下孵育2小时。通过加热去除的E受体可阻断与SRBC形成E花环，但不能阻断与AET-SRBC形成E花环。结果表明，E受体可能呈现不同的构象结构或不同的分子，分别负责E花环形成和E-AET花环形成。

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