通过用2-氨基乙基异硫脲溴化物（AET）处理的绵羊红细胞与猴红细胞形成花环来分离人T淋巴细胞和B淋巴细胞。
Isolation of human T and B lymphocytes by rosette formation with 2-aminoethylisothiquronium bromide (AET) -treated sheep red blood cells with monkey red blood cells.
作者信息
Pellegrino M A, Ferrone S, Theofilopoulos A N
出版信息
J Immunol Methods. 1976;11(3-4):273-9. doi: 10.1016/0022-1759(76)90120-4.
PMID:819587
Abstract
Purified human B and T lymphocytes were obtained by rosetting HPL with AET-SRBC or MRBC and separating the non-rosetted from the rosetted cells on Ficoll-Hypaque gradient. 92 +/- 5% of the B cells were fluorescent positive for mBlg and 95 +/- 2% of the purified T cells rosetted with AET-SRBC. 69 +/- 5% of the B cells and 61 +/- 8% of the T cells present in the unifractionated HPL were recovered in the purified fractions.
摘要
通过用AET-SRBC或MRBC对人外周血淋巴细胞（HPL）进行玫瑰花结试验，并在Ficoll-Hypaque梯度上从形成玫瑰花结的细胞中分离出未形成玫瑰花结的细胞，从而获得纯化的人B淋巴细胞和T淋巴细胞。92±5%的B细胞对mBlg呈荧光阳性，95±2%的纯化T细胞与AET-SRBC形成玫瑰花结。在未分级的HPL中存在的69±5%的B细胞和61±8%的T细胞在纯化组分中得以回收。
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