Qin Xiaojian, Li Xiaowei, Li Cuiping, Li Yuntong, Wu Qian, Wen Huan, Jiang Dan, Tang Tingting, Nan Wenbin, Liang Yongshu, Zhang Hanma
College of Life Sciences, Chongqing Normal University, Chongqing, China.
Key Laboratory of Molecular Biology of Plants Environmental Adaptations, Chongqing Normal University, Chongqing, China.
Front Plant Sci. 2023 Jun 2;14:1198809. doi: 10.3389/fpls.2023.1198809. eCollection 2023.
Rice productivity relies heavily on nitrogen fertilization, and improving nitrogen use efficiency (NUE) is important for hybrid rice breeding. Reducing nitrogen inputs is the key to achieving sustainable rice production and reducing environmental problems. Here, we analyzed the genome-wide transcriptomic changes in microRNAs (miRNAs) in the rice restorer cultivar Nanhui 511 (NH511) under high (HN) and low nitrogen (LN) conditions. The results showed that NH511 is sensitive to nitrogen supplies and HN conditions promoted the growth its lateral roots at the seedling stage. Furthermore, we identified 483 known miRNAs and 128 novel miRNAs by small RNA sequencing in response to nitrogen in NH511. We also detected 100 differentially expressed genes (DEGs), including 75 upregulated and 25 downregulated DEGs, under HN conditions. Among these DEGs, 43 miRNAs that exhibited a 2-fold change in their expression were identified in response to HN conditions, including 28 upregulated and 15 downregulated genes. Additionally, some differentially expressed miRNAs were further validated by qPCR analysis, which showed that , , and were upregulated, whereas and were downregulated under HN conditions. Moreover, the degradomes of possible target genes for and and expression variations were analyzed by qPCR at different time points under HN conditions. Our findings revealed comprehensive expression profiles of miRNAs responsive to HN treatments in an rice restorer cultivar, which advances our understanding of the regulation of nitrogen signaling mediated by miRNAs and provides novel data for high-NUE hybrid rice cultivation.
水稻产量严重依赖氮肥，提高氮素利用效率（NUE）对杂交水稻育种至关重要。减少氮投入是实现水稻可持续生产和减少环境问题的关键。在此，我们分析了水稻恢复系品种南恢511（NH511）在高氮（HN）和低氮（LN）条件下全基因组范围内微小RNA（miRNA）的转录组变化。结果表明，NH511对氮供应敏感，HN条件促进了其苗期侧根的生长。此外，我们通过小RNA测序在NH511中鉴定出483个已知miRNA和128个新miRNA对氮的响应。我们还在HN条件下检测到100个差异表达基因（DEG），包括75个上调和25个下调的DEG。在这些DEG中，鉴定出43个在HN条件下表达变化2倍的miRNA，包括28个上调和15个下调基因。此外，一些差异表达的miRNA通过qPCR分析进一步验证，结果表明，，和在HN条件下上调，而和下调。此外，在HN条件下不同时间点通过qPCR分析了和的可能靶基因的降解组及表达变化。我们的研究结果揭示了水稻恢复系品种中对HN处理响应的miRNA的全面表达谱，这加深了我们对miRNA介导的氮信号调控的理解，并为高氮素利用效率杂交水稻栽培提供了新的数据。
