Hamouda Ragaa A, Alhumairi Abrar M, Saddiq Amna A
Department of Biology, College of Sciences and Arts Khulais, University of Jeddah, Jeddah, Saudi Arabia.
Microbial Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt.
Heliyon. 2023 Jun 5;9(6):e16656. doi: 10.1016/j.heliyon.2023.e16656. eCollection 2023 Jun.
There are two major problems in the world, fuel deficiency and environmental pollution by fossil fuels. Microalgae are regarded as one of the most feasible feedstocks for the manufacturing of biofuels and are used in the degradation of fossil fuel spills. The present study was possessed to investigate the ability of green alga , blue-green alga sp, and its consortium to grow and degrade hydrocarbon such as kerosene (k) with different concentrations (0, 0.5, 1, and 1,5%), and also using algal biomasses to produce biofuel. The algal growth was estimated by optical density (O.D) at 600 nm, pigment contents such as Chlorophyll carotenoid, and dry weight. The kerosene degradation was estimated by FT-IR analysis after and before the cultivation of algae and its consortium. The components of the methanol extract were determined by GC-MS spectroscopy. The results denote the best growth was determined by O.D, algae consortium with 1.5% Kerosene after ten days, meanwhile, the highest dry weight was with after ten days of cultivation. The FT-IR demonstrated the algae and consortium possessed high efficacy to degrade kerosene. After 15 days of algae cultivation with 1% K, C.vulgaris produced the maximum amount of lipids (32%). The GC-MS profile of methanol extract of two algae and consortium demonstrated that Undecane was presented in high amounts, (19.9%), 82.16%), algae consortium (79.51%), and also were presented moderate amounts of fatty acid methyl ester in sp. Overall, our results indicate that a consortium of algae can absorb and remove kerosene from water, and at the same time produce biofuels like biodiesel and petroleum-based fuels.
世界上存在两个主要问题，即燃料短缺和化石燃料造成的环境污染。微藻被认为是制造生物燃料最可行的原料之一，并被用于降解化石燃料泄漏物。本研究旨在调查绿藻、蓝藻及其联合体在不同浓度（0、0.5%、1%和1.5%）煤油（k）环境下生长和降解碳氢化合物的能力，以及利用藻类生物质生产生物燃料的能力。通过600nm处的光密度（O.D）、叶绿素、类胡萝卜素等色素含量以及干重评估藻类生长情况。通过藻类及其联合体培养前后的傅里叶变换红外光谱（FT-IR）分析评估煤油降解情况。通过气相色谱-质谱联用（GC-MS）光谱法测定甲醇提取物的成分。结果表明，通过光密度测定，最佳生长情况是十天后含1.5%煤油的藻类联合体，同时，培养十天后干重最高。FT-IR表明藻类及其联合体具有高效降解煤油的能力。在用1%煤油培养藻类15天后，普通小球藻产生的脂质最多（32%）。两种藻类及其联合体甲醇提取物的GC-MS图谱表明，十一烷含量很高，普通小球藻中为19.9%，蓝藻中为82.16%，藻类联合体中为79.51%，同时蓝藻中脂肪酸甲酯含量适中。总体而言，我们的结果表明，藻类联合体可以从水中吸收和去除煤油，同时生产生物柴油和石油基燃料等生物燃料。
